This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1nbf
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /> <applet load="1nbf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nbf, resolution 2.3Å" /> '''Crystal structure of...)
Next diff →
Revision as of 16:13, 12 November 2007
|
Crystal structure of a UBP-family deubiquitinating enzyme in isolation and in complex with ubiquitin aldehyde
Overview
The ubiquitin-specific processing protease (UBP) family of, deubiquitinating enzymes plays an essential role in numerous cellular, processes. HAUSP, a representative UBP, specifically deubiquitinates and, hence stabilizes the tumor suppressor protein p53. Here, we report the, crystal structures of the 40 kDa catalytic core domain of HAUSP in, isolation and in complex with ubiquitin aldehyde. These studies reveal, that the UBP deubiquitinating enzymes exhibit a conserved three-domain, architecture, comprising Fingers, Palm, and Thumb. The leaving ubiquitin, moiety is specifically coordinated by the Fingers, with its C terminus, placed in the active site between the Palm and the Thumb. Binding by, ubiquitin aldehyde induces a drastic conformational change in the active, site that realigns the catalytic triad residues for catalysis.
About this Structure
1NBF is a Protein complex structure of sequences from Homo sapiens. Active as Ubiquitin thiolesterase, with EC number 3.1.2.15 Full crystallographic information is available from OCA.
Reference
Crystal structure of a UBP-family deubiquitinating enzyme in isolation and in complex with ubiquitin aldehyde., Hu M, Li P, Li M, Li W, Yao T, Wu JW, Gu W, Cohen RE, Shi Y, Cell. 2002 Dec 27;111(7):1041-54. PMID:12507430
Page seeded by OCA on Mon Nov 12 18:20:13 2007
