8ixk
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==bottom segment of the bacteriophage M13 mini variant== | |
| + | <StructureSection load='8ixk' size='340' side='right'caption='[[8ixk]], [[Resolution|resolution]] 3.30Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8ixk]] is a 25 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_M13 Escherichia virus M13]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8IXK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8IXK FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.3Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ixk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ixk OCA], [https://pdbe.org/8ixk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ixk RCSB], [https://www.ebi.ac.uk/pdbsum/8ixk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ixk ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/G3P_BPM13 G3P_BPM13] Plays essential roles both in the penetration of the viral genome into the bacterial host via pilus retraction and in the extrusion process. During the initial step of infection, G3P mediates adsorption of the phage to its primary receptor, the tip of host F-pilus. Subsequent interaction with the host entry receptor tolA induces penetration of the viral DNA into the host cytoplasm. In the extrusion process, G3P mediates the release of the membrane-anchored virion from the cell via its C-terminal domain. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Filamentous bacteriophages package their circular, single stranded DNA genome with the major coat protein pVIII and the minor coat proteins pIII, pVII, pVI, and pIX. Here, we report the cryo-EM structure of a ~500 A long bacteriophage M13 mini variant. The distal ends of the mini phage are sealed by two cap-like complexes composed of the minor coat proteins. The top cap complex consists of pVII and pIX, both exhibiting a single helix structure. Arg33 of pVII and Glu29 of pIX, located on the inner surface of the cap, play a key role in recognizing the genome packaging signal. The bottom cap complex is formed by the hook-like structures of pIII and pVI, arranged in helix barrels. Most of the inner ssDNA genome adopts a double helix structure with a similar pitch to that of the A-form double-stranded DNA. These findings provide insights into the assembly of filamentous bacteriophages. | ||
| - | + | Cryo-EM structure of a bacteriophage M13 mini variant.,Jia Q, Xiang Y Nat Commun. 2023 Sep 5;14(1):5421. doi: 10.1038/s41467-023-41151-7. PMID:37669979<ref>PMID:37669979</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Jia | + | <div class="pdbe-citations 8ixk" style="background-color:#fffaf0;"></div> |
| - | [[Category: Xiang | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Escherichia virus M13]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Jia Q]] | ||
| + | [[Category: Xiang Y]] | ||
Revision as of 18:17, 20 September 2023
bottom segment of the bacteriophage M13 mini variant
| |||||||||||
