8ixl
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==top segment of the bacteriophage M13 mini variant== | |
| + | <StructureSection load='8ixl' size='340' side='right'caption='[[8ixl]], [[Resolution|resolution]] 3.50Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8ixl]] is a 35 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_M13 Escherichia virus M13]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8IXL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8IXL FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.5Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ixl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ixl OCA], [https://pdbe.org/8ixl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ixl RCSB], [https://www.ebi.ac.uk/pdbsum/8ixl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ixl ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/CAPSD_BPM13 CAPSD_BPM13] Self assembles to form a helical capsid wrapping up the viral genomic DNA. The capsid displays a filamentous structure with a length of 760-1950 nm and a width of 6-8 nm. The virion assembly and budding take place at the host inner membrane. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Filamentous bacteriophages package their circular, single stranded DNA genome with the major coat protein pVIII and the minor coat proteins pIII, pVII, pVI, and pIX. Here, we report the cryo-EM structure of a ~500 A long bacteriophage M13 mini variant. The distal ends of the mini phage are sealed by two cap-like complexes composed of the minor coat proteins. The top cap complex consists of pVII and pIX, both exhibiting a single helix structure. Arg33 of pVII and Glu29 of pIX, located on the inner surface of the cap, play a key role in recognizing the genome packaging signal. The bottom cap complex is formed by the hook-like structures of pIII and pVI, arranged in helix barrels. Most of the inner ssDNA genome adopts a double helix structure with a similar pitch to that of the A-form double-stranded DNA. These findings provide insights into the assembly of filamentous bacteriophages. | ||
| - | + | Cryo-EM structure of a bacteriophage M13 mini variant.,Jia Q, Xiang Y Nat Commun. 2023 Sep 5;14(1):5421. doi: 10.1038/s41467-023-41151-7. PMID:37669979<ref>PMID:37669979</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Jia | + | <div class="pdbe-citations 8ixl" style="background-color:#fffaf0;"></div> |
| - | [[Category: Xiang | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Escherichia virus M13]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Jia Q]] | ||
| + | [[Category: Xiang Y]] | ||
Revision as of 18:17, 20 September 2023
top segment of the bacteriophage M13 mini variant
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