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| | <StructureSection load='5jno' size='340' side='right'caption='[[5jno]], [[Resolution|resolution]] 2.20Å' scene=''> | | <StructureSection load='5jno' size='340' side='right'caption='[[5jno]], [[Resolution|resolution]] 2.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5jno]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JNO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JNO FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5jno]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JNO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5JNO FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BEND3, KIAA1553 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), ERCC6L, PICH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_helicase DNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.12 3.6.4.12] </span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5jno FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jno OCA], [https://pdbe.org/5jno PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5jno RCSB], [https://www.ebi.ac.uk/pdbsum/5jno PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5jno ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jno FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jno OCA], [http://pdbe.org/5jno PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jno RCSB], [http://www.ebi.ac.uk/pdbsum/5jno PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jno ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/BEND3_HUMAN BEND3_HUMAN]] Transcriptional repressor which associates with the NoRC (nucleolar remodeling complex) complex and plays a key role in repressing rDNA transcription. The sumoylated form modulates the stability of the NoRC complex component BAZ2A/TIP5 by controlling its USP21-mediated deubiquitination (PubMed:21914818, PubMed:26100909). Binds to unmethylated major satellite DNA and is involved in the recruitment of the Polycomb repressive complex 2 (PRC2) to major satellites (By similarity).[UniProtKB:Q6PAL0]<ref>PMID:21914818</ref> <ref>PMID:26100909</ref> [[http://www.uniprot.org/uniprot/ERC6L_HUMAN ERC6L_HUMAN]] DNA helicase that acts as an essential component of the spindle assembly checkpoint. Contributes to the mitotic checkpoint by recruiting MAD2 to kinetochores and monitoring tension on centromeric chromatin. Acts as a tension sensor that associates with catenated DNA which is stretched under tension until it is resolved during anaphase.<ref>PMID:17218258</ref> | + | [https://www.uniprot.org/uniprot/BEND3_HUMAN BEND3_HUMAN] Transcriptional repressor which associates with the NoRC (nucleolar remodeling complex) complex and plays a key role in repressing rDNA transcription. The sumoylated form modulates the stability of the NoRC complex component BAZ2A/TIP5 by controlling its USP21-mediated deubiquitination (PubMed:21914818, PubMed:26100909). Binds to unmethylated major satellite DNA and is involved in the recruitment of the Polycomb repressive complex 2 (PRC2) to major satellites (By similarity).[UniProtKB:Q6PAL0]<ref>PMID:21914818</ref> <ref>PMID:26100909</ref> |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: DNA helicase]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Human]]
| + | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Hickson, I D]] | + | [[Category: Hickson ID]] |
| - | [[Category: Mesa, P]] | + | [[Category: Mesa P]] |
| - | [[Category: Montoya, G]] | + | [[Category: Montoya G]] |
| - | [[Category: Pitchai, G]] | + | [[Category: Pitchai G]] |
| - | [[Category: Anaphase]]
| + | |
| - | [[Category: Cell cycle]]
| + | |
| - | [[Category: Sister chromatids separation]]
| + | |
| - | [[Category: Ultra fine chromatin bridge]]
| + | |
| Structural highlights
Function
BEND3_HUMAN Transcriptional repressor which associates with the NoRC (nucleolar remodeling complex) complex and plays a key role in repressing rDNA transcription. The sumoylated form modulates the stability of the NoRC complex component BAZ2A/TIP5 by controlling its USP21-mediated deubiquitination (PubMed:21914818, PubMed:26100909). Binds to unmethylated major satellite DNA and is involved in the recruitment of the Polycomb repressive complex 2 (PRC2) to major satellites (By similarity).[UniProtKB:Q6PAL0][1] [2]
References
- ↑ Sathyan KM, Shen Z, Tripathi V, Prasanth KV, Prasanth SG. A BEN-domain-containing protein associates with heterochromatin and represses transcription. J Cell Sci. 2011 Sep 15;124(Pt 18):3149-63. doi: 10.1242/jcs.086603. PMID:21914818 doi:http://dx.doi.org/10.1242/jcs.086603
- ↑ Khan A, Giri S, Wang Y, Chakraborty A, Ghosh AK, Anantharaman A, Aggarwal V, Sathyan KM, Ha T, Prasanth KV, Prasanth SG. BEND3 represses rDNA transcription by stabilizing a NoRC component via USP21 deubiquitinase. Proc Natl Acad Sci U S A. 2015 Jul 7;112(27):8338-43. doi:, 10.1073/pnas.1424705112. Epub 2015 Jun 22. PMID:26100909 doi:http://dx.doi.org/10.1073/pnas.1424705112
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