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| | <StructureSection load='5jr6' size='340' side='right'caption='[[5jr6]], [[Resolution|resolution]] 2.30Å' scene=''> | | <StructureSection load='5jr6' size='340' side='right'caption='[[5jr6]], [[Resolution|resolution]] 2.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5jr6]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Plaf7 Plaf7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JR6 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5JR6 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5jr6]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum_3D7 Plasmodium falciparum 3D7] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JR6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5JR6 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=01B:(2S,3R)-3-AMINO-2-HYDROXY-4-PHENYLBUTANOIC+ACID'>01B</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=01B:(2S,3R)-3-AMINO-2-HYDROXY-4-PHENYLBUTANOIC+ACID'>01B</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5jqk|5jqk]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5jr6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jr6 OCA], [https://pdbe.org/5jr6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5jr6 RCSB], [https://www.ebi.ac.uk/pdbsum/5jr6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5jr6 ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PF14_0517 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36329 PLAF7])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5jr6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jr6 OCA], [http://pdbe.org/5jr6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jr6 RCSB], [http://www.ebi.ac.uk/pdbsum/5jr6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jr6 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0A144A2H0_PLAF7 A0A144A2H0_PLAF7] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Plaf7]] | + | [[Category: Plasmodium falciparum 3D7]] |
| - | [[Category: Drinkwater, N]] | + | [[Category: Synthetic construct]] |
| - | [[Category: McGowan, S]] | + | [[Category: Drinkwater N]] |
| - | [[Category: Aminopeptidase]] | + | [[Category: McGowan S]] |
| - | [[Category: Hydrolase]]
| + | |
| Structural highlights
Function
A0A144A2H0_PLAF7
Publication Abstract from PubMed
Malaria is one of the world's most prevalent parasitic diseases, with over 200 million cases annually. Alarmingly, the spread of drug-resistant parasites threatens the effectiveness of current antimalarials, and has made the development of novel therapeutic strategies a global health priority. Malaria parasites have a complicated lifecycle involving an asymptomatic 'liver stage' and a symptomatic 'blood stage'. During the blood stage, the parasites utilise a proteolytic cascade to digest host haemoglobin, which produces free amino acids absolutely necessary for parasite growth and reproduction. The enzymes required for haemoglobin digestion are therefore attractive therapeutic targets. The final step of the cascade is catalyzed by several metalloaminopeptidases (MAPs), including aminopeptidase P (APP). We developed a novel platform to examine the substrate fingerprint of APP from P. falciparum ( Pf APP), and show that it can catalyse the removal of any residue immediately prior to a proline. Further, we have determined the crystal structure of Pf APP, and present the first examination of the 3D structure of this essential malarial enzyme. Together, these analyses provide insight into potential mechanisms of inhibition that could be used to develop novel antimalarial therapeutics.
Structure and Substrate Fingerprint of Aminopeptidase P from Plasmodium falciparum.,Drinkwater N, Kannan Sivaraman K, Bamert RS, Rut W, Mohamed K, Vinh NB, Scammells PJ, Drag M, McGowan S Biochem J. 2016 Jul 26. pii: BCJ20160550. PMID:27462122[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Drinkwater N, Kannan Sivaraman K, Bamert RS, Rut W, Mohamed K, Vinh NB, Scammells PJ, Drag M, McGowan S. Structure and Substrate Fingerprint of Aminopeptidase P from Plasmodium falciparum. Biochem J. 2016 Jul 26. pii: BCJ20160550. PMID:27462122 doi:http://dx.doi.org/10.1042/BCJ20160550
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