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| | <StructureSection load='5jys' size='340' side='right'caption='[[5jys]], [[Resolution|resolution]] 1.90Å' scene=''> | | <StructureSection load='5jys' size='340' side='right'caption='[[5jys]], [[Resolution|resolution]] 1.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5jys]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JYS OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5JYS FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5jys]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JYS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5JYS FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.901Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5ete|5ete]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PRY1, YJL079C, J1022 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5jys FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jys OCA], [https://pdbe.org/5jys PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5jys RCSB], [https://www.ebi.ac.uk/pdbsum/5jys PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5jys ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5jys FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jys OCA], [http://pdbe.org/5jys PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jys RCSB], [http://www.ebi.ac.uk/pdbsum/5jys PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jys ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/PRY1_YEAST PRY1_YEAST]] Secreted protein required for efficient export of lipids such as acetylated sterols. Acts in detoxification of hydrophobic compounds.<ref>PMID:23027975</ref> | + | [https://www.uniprot.org/uniprot/PRY1_YEAST PRY1_YEAST] Secreted protein required for efficient export of lipids such as acetylated sterols. Acts in detoxification of hydrophobic compounds.<ref>PMID:23027975</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Baker's yeast]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Asojo, O A]] | + | [[Category: Saccharomyces cerevisiae S288C]] |
| - | [[Category: Cap protein]] | + | [[Category: Asojo OA]] |
| - | [[Category: Transport protein]]
| + | |
| Structural highlights
Function
PRY1_YEAST Secreted protein required for efficient export of lipids such as acetylated sterols. Acts in detoxification of hydrophobic compounds.[1]
Publication Abstract from PubMed
The production, crystal structure, and functional characterization of the C-terminal cysteine-rich secretory protein/antigen 5/pathogenesis related-1 (CAP) domain of pathogen-related yeast protein-1 (Pry1) from Saccharomyces cerevisiae is presented. The CAP domain of Pry1 (Pry1CAP) is functional in vivo as its expression restores cholesterol export to yeast mutants lacking endogenous Pry1 and Pry2. Recombinant Pry1CAP forms dimers in solution, is sufficient for in vitro cholesterol binding, and has comparable binding properties as full-length Pry1. Two crystal structures of Pry1CAP are reported, one with Mg(2+) coordinated to the conserved CAP tetrad (His208, Glu215, Glu233 and His250) in spacegroup I41 and the other without divalent cations in spacegroup P6122. The latter structure contains four 1,4-dioxane molecules from the crystallization solution, one of which sits in the cholesterol binding site. Both structures reveal that the divalent cation and cholesterol binding sites are connected upon dimerization, providing a structural basis for the observed Mg(2+)-dependent sterol binding by Pry1.
Structural and functional characterization of the CAP domain of pathogen-related yeast 1 (Pry1) protein.,Darwiche R, Kelleher A, Hudspeth EM, Schneiter R, Asojo OA Sci Rep. 2016 Jun 27;6:28838. doi: 10.1038/srep28838. PMID:27344972[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Choudhary V, Schneiter R. Pathogen-Related Yeast (PRY) proteins and members of the CAP superfamily are secreted sterol-binding proteins. Proc Natl Acad Sci U S A. 2012 Oct 16;109(42):16882-7. doi:, 10.1073/pnas.1209086109. Epub 2012 Oct 1. PMID:23027975 doi:http://dx.doi.org/10.1073/pnas.1209086109
- ↑ Darwiche R, Kelleher A, Hudspeth EM, Schneiter R, Asojo OA. Structural and functional characterization of the CAP domain of pathogen-related yeast 1 (Pry1) protein. Sci Rep. 2016 Jun 27;6:28838. doi: 10.1038/srep28838. PMID:27344972 doi:http://dx.doi.org/10.1038/srep28838
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