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| | <StructureSection load='5k1q' size='340' side='right'caption='[[5k1q]], [[Resolution|resolution]] 1.55Å' scene=''> | | <StructureSection load='5k1q' size='340' side='right'caption='[[5k1q]], [[Resolution|resolution]] 1.55Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5k1q]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sheon Sheon]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K1Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5K1Q FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5k1q]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Shewanella_oneidensis_MR-1 Shewanella oneidensis MR-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K1Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5K1Q FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=PCR:P-CRESOL'>PCR</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">sye4, SO_3392 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=211586 SHEON])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=PCR:P-CRESOL'>PCR</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Oxidoreductase Oxidoreductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.30 1.5.1.30] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5k1q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k1q OCA], [https://pdbe.org/5k1q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5k1q RCSB], [https://www.ebi.ac.uk/pdbsum/5k1q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5k1q ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5k1q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k1q OCA], [http://pdbe.org/5k1q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5k1q RCSB], [http://www.ebi.ac.uk/pdbsum/5k1q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5k1q ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q8EBV3_SHEON Q8EBV3_SHEON] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Oxidoreductase]] | + | [[Category: Shewanella oneidensis MR-1]] |
| - | [[Category: Sheon]]
| + | [[Category: Brige A]] |
| - | [[Category: Brige, A]] | + | [[Category: Elegheert J]] |
| - | [[Category: Elegheert, J]] | + | [[Category: Savvides SN]] |
| - | [[Category: Savvides, S N]] | + | |
| - | [[Category: Cofactor-binding]]
| + | |
| Structural highlights
Function
Q8EBV3_SHEON
Publication Abstract from PubMed
Shewanella oneidensis, a Gram-negative gamma-proteobacterium with an extensive redox capacity, possesses four old yellow enzyme (OYE) homologs. Of these, Shewanella yellow enzyme 4 (SYE4) is implicated in resistance to oxidative stress. Here, we present a series of high-resolution crystal structures for SYE4 in the oxidized and reduced states, and in complex with phenolic ligands and the nitro-aromatic explosive picric acid. The structures unmask new features, including the identification of a binding platform for long-chain hydrophobic molecules. Furthermore, we present the first structural observation of a hydride-Meisenheimer complex of picric acid with a flavoenzyme. Overall, our study exposes the binding promiscuity of SYE4 toward a variety of electrophilic substrates and is consistent with a general detoxification function for SYE4.
Structural dissection of Shewanella oneidensis old yellow enzyme 4 bound to a Meisenheimer complex and (nitro)phenolic ligands.,Elegheert J, Brige A, Van Beeumen J, Savvides SN FEBS Lett. 2017 Sep 4. doi: 10.1002/1873-3468.12833. PMID:28869767[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Elegheert J, Brige A, Van Beeumen J, Savvides SN. Structural dissection of Shewanella oneidensis old yellow enzyme 4 bound to a Meisenheimer complex and (nitro)phenolic ligands. FEBS Lett. 2017 Sep 4. doi: 10.1002/1873-3468.12833. PMID:28869767 doi:http://dx.doi.org/10.1002/1873-3468.12833
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