1n0i
From Proteopedia
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'''Crystal Structure of Ferrochelatase with Cadmium bound at active site''' | '''Crystal Structure of Ferrochelatase with Cadmium bound at active site''' | ||
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[[Category: Ryde, U.]] | [[Category: Ryde, U.]] | ||
[[Category: Sigfridsson, E.]] | [[Category: Sigfridsson, E.]] | ||
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Revision as of 22:56, 2 May 2008
Crystal Structure of Ferrochelatase with Cadmium bound at active site
Overview
Ferrochelatase, the terminal enzyme in heme biosynthesis, catalyses metal insertion into protoporphyrin IX. The location of the metal binding site with respect to the bound porphyrin substrate and the mode of metal binding are of central importance for understanding the mechanism of porphyrin metallation. In this work we demonstrate that Zn(2+), which is commonly used as substrate in assays of the ferrochelatase reaction, and Cd(2+), an inhibitor of the enzyme, bind to the invariant amino acids His183 and Glu264 and water molecules, all located within the porphyrin binding cleft. On the other hand, Mg(2+), which has been shown to bind close to the surface at 7 A from His183, was largely absent from its site. Activity measurements demonstrate that Mg(2+) has a stimulatory effect on the enzyme, lowering K(M) for Zn(2+) from 55 to 24 micro M. Changing one of the Mg(2+) binding residues, Glu272, to serine abolishes the effect of Mg(2+). It is proposed that prior to metal insertion the metal may form a sitting-atop (SAT) complex with the invariant His-Glu couple and the porphyrin. Metal binding to the Mg(2+) site may stimulate metal release from the protein ligands and its insertion into the porphyrin.
About this Structure
1N0I is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.
Reference
Metal binding to Bacillus subtilis ferrochelatase and interaction between metal sites., Lecerof D, Fodje MN, Alvarez Leon R, Olsson U, Hansson A, Sigfridsson E, Ryde U, Hansson M, Al-Karadaghi S, J Biol Inorg Chem. 2003 Apr;8(4):452-8. Epub 2003 Jan 18. PMID:12761666 Page seeded by OCA on Sat May 3 01:56:08 2008
