1n1a
From Proteopedia
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'''Crystal Structure of the N-terminal domain of human FKBP52''' | '''Crystal Structure of the N-terminal domain of human FKBP52''' | ||
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[[Category: Shu, C.]] | [[Category: Shu, C.]] | ||
[[Category: Wu, B.]] | [[Category: Wu, B.]] | ||
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| - | [[Category: | + | [[Category: Fkbp52]] |
| - | [[Category: | + | [[Category: The n-terminal domain]] |
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Revision as of 22:57, 2 May 2008
Crystal Structure of the N-terminal domain of human FKBP52
Overview
FKBP52 is a member of the FK506-binding protein family (FKBPs). The N-terminal domain of FKBP52 (FKBP52-N; residues 1-140) is responsible for peptidyl-prolyl isomerase activity and binding of FK506. Here, the crystal structure of FKBP52-N has been determined by molecular replacement to 2.4 A. FKBP52-N is defined by a six-stranded antiparallel beta-sheet wrapping with a right-handed twist around a short alpha-helix, an architecture similar to that of FKBP12. FKBP52-N is able to bind FK506 in a similar way to FKBP12. The variability in two loop regions (residues 70-76 and 108-127) is the principal reason for the specificity differences between FKBP52-N and FKBP12. The Pro120 change corresponding to Gly89 in FKBP12 limits the conformational adaptation between the loop (residues 108-127) and FK506 and decreases the FK506 affinity, while the Lys121 substitution corresponding to Ile90 of FKBP12 destroys a key interaction between FKBP52-N and calcineurin. It can be inferred from the locations of strictly conserved amino acids in the polypeptide chain that the maintenance of the overall conformation of the PPIase domains of FKBPs is essential for the PPIase activity. The N-terminal region and beta-sheets of FKBP52-N forms a hydrophobic patch which may be responsible for the binding of target proteins such as dynein or PAHX.
About this Structure
1N1A is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure of the N-terminal domain of human FKBP52., Li P, Ding Y, Wu B, Shu C, Shen B, Rao Z, Acta Crystallogr D Biol Crystallogr. 2003 Jan;59(Pt 1):16-22. Epub 2002, Dec 19. PMID:12499534 Page seeded by OCA on Sat May 3 01:57:49 2008
