1n2m
From Proteopedia
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'''The S53A Proenzyme Structure of Methanococcus jannaschii.''' | '''The S53A Proenzyme Structure of Methanococcus jannaschii.''' | ||
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[[Category: Tolbert, W D.]] | [[Category: Tolbert, W D.]] | ||
[[Category: White, R H.]] | [[Category: White, R H.]] | ||
| - | [[Category: | + | [[Category: Agmatine]] |
| - | [[Category: | + | [[Category: Arginine decarboxylase]] |
| - | [[Category: | + | [[Category: Pyruvate]] |
| - | [[Category: | + | [[Category: Pyruvoyl group]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:00:50 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 23:00, 2 May 2008
The S53A Proenzyme Structure of Methanococcus jannaschii.
Overview
The three-dimensional structure of pyruvoyl-dependent arginine decarboxylase from Methanococcus jannaschii was determined at 1.4 A resolution. The pyruvoyl group of arginine decarboxylase is generated by an autocatalytic internal serinolysis reaction at Ser53 in the proenzyme resulting in two polypeptide chains. The structure of the nonprocessing S53A mutant was also determined. The active site of the processed enzyme unexpectedly contained the reaction product agmatine. The crystal structure confirms that arginine decarboxylase is a homotrimer. The protomer fold is a four-layer alphabetabetaalpha sandwich with topology similar to pyruvoyl-dependent histidine decarboxylase. Highly conserved residues Asn47, Ser52, Ser53, Ile54, and Glu109 are proposed to play roles in the self-processing reaction. Agmatine binding residues include the C terminus of the beta chain (Ser52) from one protomer and the Asp35 side chain and the Gly44 and Val46 carbonyl oxygen atoms from an adjacent protomer. Glu109 is proposed to play a catalytic role in the decarboxylation reaction.
About this Structure
1N2M is a Single protein structure of sequence from Methanocaldococcus jannaschii. Full crystallographic information is available from OCA.
Reference
Pyruvoyl-dependent arginine decarboxylase from Methanococcus jannaschii: crystal structures of the self-cleaved and S53A proenzyme forms., Tolbert WD, Graham DE, White RH, Ealick SE, Structure. 2003 Mar;11(3):285-94. PMID:12623016 Page seeded by OCA on Sat May 3 02:00:50 2008
