This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1n32

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:1n32.gif|left|200px]]
[[Image:1n32.gif|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 1n32 |SIZE=350|CAPTION= <scene name='initialview01'>1n32</scene>, resolution 3.00&Aring;
+
The line below this paragraph, containing "STRUCTURE_1n32", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PAR:PAROMOMYCIN'>PAR</scene>, <scene name='pdbligand=PSU:PSEUDOURIDINE-5&#39;-MONOPHOSPHATE'>PSU</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY=
+
or leave the SCENE parameter empty for the default display.
-
|GENE=
+
-->
-
|DOMAIN=
+
{{STRUCTURE_1n32| PDB=1n32 | SCENE= }}
-
|RELATEDENTRY=[[1j5e|1J5E]], [[1fjg|1FJG]], [[1ibl|1IBL]], [[1ibk|1IBK]], [[1ibm|1IBM]], [[1n33|1N33]], [[1n34|1N34]], [[1n36|1N36]]
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n32 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n32 OCA], [http://www.ebi.ac.uk/pdbsum/1n32 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1n32 RCSB]</span>
+
-
}}
+
'''Structure of the Thermus thermophilus 30S ribosomal subunit bound to codon and near-cognate transfer RNA anticodon stem-loop mismatched at the first codon position at the a site with paromomycin'''
'''Structure of the Thermus thermophilus 30S ribosomal subunit bound to codon and near-cognate transfer RNA anticodon stem-loop mismatched at the first codon position at the a site with paromomycin'''
Line 30: Line 27:
[[Category: Tarry, M J.]]
[[Category: Tarry, M J.]]
[[Category: 30s ribosomal subunit]]
[[Category: 30s ribosomal subunit]]
-
[[Category: a site]]
+
[[Category: A site]]
-
[[Category: antibiotic]]
+
[[Category: Antibiotic]]
-
[[Category: anticodon]]
+
[[Category: Anticodon]]
-
[[Category: codon]]
+
[[Category: Codon]]
-
[[Category: decoding]]
+
[[Category: Decoding]]
-
[[Category: g:u]]
+
[[Category: G:u]]
-
[[Category: gu]]
+
[[Category: Gu]]
-
[[Category: messenger rna]]
+
[[Category: Messenger rna]]
-
[[Category: mismatch]]
+
[[Category: Mismatch]]
-
[[Category: mrna]]
+
[[Category: Mrna]]
-
[[Category: near-cognate]]
+
[[Category: Near-cognate]]
-
[[Category: paromomycin]]
+
[[Category: Paromomycin]]
-
[[Category: ribosome]]
+
[[Category: Ribosome]]
-
[[Category: stem-loop]]
+
[[Category: Stem-loop]]
-
[[Category: transfer rna]]
+
[[Category: Transfer rna]]
-
[[Category: trna]]
+
[[Category: Trna]]
-
[[Category: wobble]]
+
[[Category: Wobble]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:01:37 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:24:39 2008''
+

Revision as of 23:01, 2 May 2008

Image:1n32.gif

Template:STRUCTURE 1n32

Structure of the Thermus thermophilus 30S ribosomal subunit bound to codon and near-cognate transfer RNA anticodon stem-loop mismatched at the first codon position at the a site with paromomycin


Overview

A structural and mechanistic explanation for the selection of tRNAs by the ribosome has been elusive. Here, we report crystal structures of the 30S ribosomal subunit with codon and near-cognate tRNA anticodon stem loops bound at the decoding center and compare affinities of equivalent complexes in solution. In ribosomal interactions with near-cognate tRNA, deviation from Watson-Crick geometry results in uncompensated desolvation of hydrogen-bonding partners at the codon-anticodon minor groove. As a result, the transition to a closed form of the 30S induced by cognate tRNA is unfavorable for near-cognate tRNA unless paromomycin induces part of the rearrangement. We conclude that stabilization of a closed 30S conformation is required for tRNA selection, and thereby structurally rationalize much previous data on translational fidelity.

About this Structure

1N32 is a Protein complex structure of sequences from Thermus thermophilus. Full crystallographic information is available from OCA.

Reference

Selection of tRNA by the ribosome requires a transition from an open to a closed form., Ogle JM, Murphy FV, Tarry MJ, Ramakrishnan V, Cell. 2002 Nov 27;111(5):721-32. PMID:12464183 Page seeded by OCA on Sat May 3 02:01:37 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1n32"
Personal tools