1n33

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[[Image:1n33.gif|left|200px]]
[[Image:1n33.gif|left|200px]]
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{{Structure
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|PDB= 1n33 |SIZE=350|CAPTION= <scene name='initialview01'>1n33</scene>, resolution 3.35&Aring;
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|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PAR:PAROMOMYCIN'>PAR</scene>, <scene name='pdbligand=PSU:PSEUDOURIDINE-5&#39;-MONOPHOSPHATE'>PSU</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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{{STRUCTURE_1n33| PDB=1n33 | SCENE= }}
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|RELATEDENTRY=[[1j5e|1J5E]], [[1fjg|1FJG]], [[1ibl|1IBL]], [[1ibk|1IBK]], [[1ibm|1IBM]], [[1n32|1N32]], [[1n34|1N34]], [[1n36|1N36]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n33 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n33 OCA], [http://www.ebi.ac.uk/pdbsum/1n33 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1n33 RCSB]</span>
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'''Structure of the Thermus thermophilus 30S ribosomal subunit bound to codon and near-cognate transfer rna anticodon stem-loop mismatched at the second codon position at the a site with paromomycin'''
'''Structure of the Thermus thermophilus 30S ribosomal subunit bound to codon and near-cognate transfer rna anticodon stem-loop mismatched at the second codon position at the a site with paromomycin'''
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[[Category: Tarry, M J.]]
[[Category: Tarry, M J.]]
[[Category: 30s ribosomal subunit]]
[[Category: 30s ribosomal subunit]]
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[[Category: a site]]
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[[Category: A site]]
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[[Category: antibiotic]]
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[[Category: Antibiotic]]
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[[Category: anticodon]]
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[[Category: Anticodon]]
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[[Category: codon]]
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[[Category: Codon]]
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[[Category: decoding]]
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[[Category: Decoding]]
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[[Category: g:u]]
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[[Category: G:u]]
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[[Category: gu]]
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[[Category: Gu]]
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[[Category: messenger rna]]
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[[Category: Messenger rna]]
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[[Category: mismatch]]
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[[Category: Mismatch]]
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[[Category: mrna]]
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[[Category: Mrna]]
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[[Category: near-cognate]]
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[[Category: Near-cognate]]
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[[Category: paromomycin]]
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[[Category: Paromomycin]]
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[[Category: ribosome]]
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[[Category: Ribosome]]
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[[Category: stem-loop]]
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[[Category: Stem-loop]]
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[[Category: transfer rna]]
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[[Category: Transfer rna]]
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[[Category: trna]]
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[[Category: Trna]]
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[[Category: wobble]]
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[[Category: Wobble]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:01:48 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:24:42 2008''
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Revision as of 23:01, 2 May 2008

Image:1n33.gif

Template:STRUCTURE 1n33

Structure of the Thermus thermophilus 30S ribosomal subunit bound to codon and near-cognate transfer rna anticodon stem-loop mismatched at the second codon position at the a site with paromomycin


Overview

A structural and mechanistic explanation for the selection of tRNAs by the ribosome has been elusive. Here, we report crystal structures of the 30S ribosomal subunit with codon and near-cognate tRNA anticodon stem loops bound at the decoding center and compare affinities of equivalent complexes in solution. In ribosomal interactions with near-cognate tRNA, deviation from Watson-Crick geometry results in uncompensated desolvation of hydrogen-bonding partners at the codon-anticodon minor groove. As a result, the transition to a closed form of the 30S induced by cognate tRNA is unfavorable for near-cognate tRNA unless paromomycin induces part of the rearrangement. We conclude that stabilization of a closed 30S conformation is required for tRNA selection, and thereby structurally rationalize much previous data on translational fidelity.

About this Structure

1N33 is a Protein complex structure of sequences from Thermus thermophilus. Full crystallographic information is available from OCA.

Reference

Selection of tRNA by the ribosome requires a transition from an open to a closed form., Ogle JM, Murphy FV, Tarry MJ, Ramakrishnan V, Cell. 2002 Nov 27;111(5):721-32. PMID:12464183 Page seeded by OCA on Sat May 3 02:01:48 2008

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