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| | <StructureSection load='5k5d' size='340' side='right'caption='[[5k5d]], [[Resolution|resolution]] 2.45Å' scene=''> | | <StructureSection load='5k5d' size='340' side='right'caption='[[5k5d]], [[Resolution|resolution]] 2.45Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5k5d]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Sacs9 Sacs9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K5D OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5K5D FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5k5d]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus_98/2 Saccharolobus solfataricus 98/2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K5D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5K5D FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.45Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5k5a|5k5a]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5k5d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k5d OCA], [https://pdbe.org/5k5d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5k5d RCSB], [https://www.ebi.ac.uk/pdbsum/5k5d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5k5d ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Ssol_1539 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=555311 SACS9])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5k5d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k5d OCA], [http://pdbe.org/5k5d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5k5d RCSB], [http://www.ebi.ac.uk/pdbsum/5k5d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5k5d ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/D0KSP7_SACS9 D0KSP7_SACS9] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Sacs9]] | + | [[Category: Saccharolobus solfataricus 98/2]] |
| - | [[Category: Schumacher, M]] | + | [[Category: Schumacher M]] |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Parb-n]]
| + | |
| - | [[Category: Partition]]
| + | |
| - | [[Category: Pnob8]]
| + | |
| Structural highlights
Function
D0KSP7_SACS9
Publication Abstract from PubMed
Although recent studies have provided a wealth of information about archaeal biology, nothing is known about the molecular basis of DNA segregation in these organisms. Here, we unveil the machinery and assembly mechanism of the archaeal Sulfolobus pNOB8 partition system. This system uses three proteins: ParA; an atypical ParB adaptor; and a centromere-binding component, AspA. AspA utilizes a spreading mechanism to create a DNA superhelix onto which ParB assembles. This supercomplex links to the ParA motor, which contains a bacteria-like Walker motif. The C domain of ParB harbors structural similarity to CenpA, which dictates eukaryotic segregation. Thus, this archaeal system combines bacteria-like and eukarya-like components, which suggests the possible conservation of DNA segregation principles across the three domains of life.
Structures of archaeal DNA segregation machinery reveal bacterial and eukaryotic linkages.,Schumacher MA, Tonthat NK, Lee J, Rodriguez-Castaneda FA, Chinnam NB, Kalliomaa-Sanford AK, Ng IW, Barge MT, Shaw PL, Barilla D Science. 2015 Sep 4;349(6252):1120-4. doi: 10.1126/science.aaa9046. PMID:26339031[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Schumacher MA, Tonthat NK, Lee J, Rodriguez-Castaneda FA, Chinnam NB, Kalliomaa-Sanford AK, Ng IW, Barge MT, Shaw PL, Barilla D. Structures of archaeal DNA segregation machinery reveal bacterial and eukaryotic linkages. Science. 2015 Sep 4;349(6252):1120-4. doi: 10.1126/science.aaa9046. PMID:26339031 doi:http://dx.doi.org/10.1126/science.aaa9046
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