|
|
| Line 3: |
Line 3: |
| | <StructureSection load='5khl' size='340' side='right'caption='[[5khl]], [[Resolution|resolution]] 2.40Å' scene=''> | | <StructureSection load='5khl' size='340' side='right'caption='[[5khl]], [[Resolution|resolution]] 2.40Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5khl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibc3 Vibc3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KHL OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5KHL FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5khl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae_O395 Vibrio cholerae O395]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KHL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5KHL FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.397Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">hutB, VC0395_0324 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=345073 VIBC3])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5khl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5khl OCA], [http://pdbe.org/5khl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5khl RCSB], [http://www.ebi.ac.uk/pdbsum/5khl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5khl ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5khl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5khl OCA], [https://pdbe.org/5khl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5khl RCSB], [https://www.ebi.ac.uk/pdbsum/5khl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5khl ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/A0A0H3AE70_VIBC3 A0A0H3AE70_VIBC3] |
| - | The mechanism of haem transport across the inner membrane of pathogenic bacteria is currently insufficiently understood at the molecular level and no information is available for this process in Vibrio cholerae. To obtain structural insights into the periplasmic haem-binding protein HutB from V. cholerae (VcHutB), which is involved in haem transport through the HutBCD haem-transport system, at the atomic level, VcHutB was cloned, overexpressed and crystallized using 1.6 M ammonium sulfate as a precipitant at pH 7.0. X-ray diffraction data were collected to 2.4 A resolution on the RRCAT PX-BL-21 beamline at the Indus-2 synchrotron, Indore, India. The crystals belonged to space group P4(3)2(1)2, with unit-cell parameters a = b = 62.88, c = 135.8 A. Matthews coefficient calculations indicated the presence of one monomer in the asymmetric unit, with an approximate solvent content of 45.02%. Molecular-replacement calculations with Phaser confirmed the presence of a monomer in the asymmetric unit.
| + | |
| - | | + | |
| - | Purification, crystallization and preliminary X-ray analysis of the periplasmic haem-binding protein HutB from Vibrio cholerae.,Agarwal S, Biswas M, Dasgupta J Acta Crystallogr F Struct Biol Commun. 2015 Apr;71(Pt 4):401-4. doi:, 10.1107/S2053230X15003660. Epub 2015 Mar 20. PMID:25849499<ref>PMID:25849499</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 5khl" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Vibc3]] | + | [[Category: Vibrio cholerae O395]] |
| - | [[Category: Agarwal, S]] | + | [[Category: Agarwal S]] |
| - | [[Category: Dasgupta, J]] | + | [[Category: Dasgupta J]] |
| - | [[Category: Dey, S]] | + | [[Category: Dey S]] |
| - | [[Category: Ghosh, B]] | + | [[Category: Ghosh B]] |
| - | [[Category: Abc trasporter]]
| + | |
| - | [[Category: Hemin]]
| + | |
| - | [[Category: Periplasmic protein]]
| + | |
| - | [[Category: Transport protein]]
| + | |
| - | [[Category: Vibrio cholerae]]
| + | |
