1n51

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[[Image:1n51.gif|left|200px]]
[[Image:1n51.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1n51 |SIZE=350|CAPTION= <scene name='initialview01'>1n51</scene>, resolution 2.30&Aring;
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The line below this paragraph, containing "STRUCTURE_1n51", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=ATN:N-[(2S,3R)-3-AMINO-2-HYDROXY-4-PHENYL-BUTANOYL]-L-PROLYL-L-PROLYL-L-ALANINAMIDE'>ATN</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Xaa-Pro_aminopeptidase Xaa-Pro aminopeptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.11.9 3.4.11.9] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= PEPP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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|DOMAIN=
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{{STRUCTURE_1n51| PDB=1n51 | SCENE= }}
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|RELATEDENTRY=[[1az9|1AZ9]], [[1a16|1A16]], [[1jaw|1JAW]], [[1m35|1M35]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n51 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n51 OCA], [http://www.ebi.ac.uk/pdbsum/1n51 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1n51 RCSB]</span>
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}}
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'''Aminopeptidase P in complex with the inhibitor apstatin'''
'''Aminopeptidase P in complex with the inhibitor apstatin'''
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[[Category: Maher, M J.]]
[[Category: Maher, M J.]]
[[Category: Simmons, W H.]]
[[Category: Simmons, W H.]]
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[[Category: aminopeptidase]]
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[[Category: Aminopeptidase]]
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[[Category: inhibitor complex]]
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[[Category: Inhibitor complex]]
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[[Category: manganese enzyme]]
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[[Category: Manganese enzyme]]
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[[Category: proline specific]]
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[[Category: Proline specific]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:06:15 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:25:27 2008''
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Revision as of 23:06, 2 May 2008

Image:1n51.gif

Template:STRUCTURE 1n51

Aminopeptidase P in complex with the inhibitor apstatin


Overview

Aminopeptidase P (APPro) is a metalloprotease whose active site includes a dinuclear manganese(II) cluster. The enzyme cleaves the N-terminal residue from a polypeptide when the second residue is proline. A complex of Escherichia coli APPro (EcAPPro) with an inhibitor, apstatin [N-(2S,3R)-3-amino-2-hydroxy-4-phenyl-butanoyl-L-prolyl-L-prolyl-L-alanina mide], has been crystallized. Apstatin binds to the active site of EcAPPro with its N-terminal amino group coordinated to one of the two Mn(II) atoms at the metal centre. The apstatin hydroxyl group replaces a hydroxide ion which bridges the two metal atoms in the native enzyme. The first proline residue of apstatin lies in a small hydrophobic cleft. The structure of the apstatin-EcAPPro complex has been refined at 2.3 A resolution with residuals R = 0.179 and R(free) = 0.204. The structure of the complex illustrates how apstatin inhibits APPro and suggests how substrates may bind to the enzyme, but the basis of the proline-specificity remains elusive.

About this Structure

1N51 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structure of Escherichia coli aminopeptidase P in complex with the inhibitor apstatin., Graham SC, Maher MJ, Simmons WH, Freeman HC, Guss JM, Acta Crystallogr D Biol Crystallogr. 2004 Oct;60(Pt 10):1770-9. Epub 2004, Sep 23. PMID:15388923 Page seeded by OCA on Sat May 3 02:06:15 2008

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OCA

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