8ca9
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Cryo-EM structure of the Cibeles-Demetra 3:3 heterocomplex from Galleria mellonella saliva== | |
| + | <StructureSection load='8ca9' size='340' side='right'caption='[[8ca9]], [[Resolution|resolution]] 2.29Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8ca9]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Galleria_mellonella Galleria mellonella]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8CA9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8CA9 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.29Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ca9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ca9 OCA], [https://pdbe.org/8ca9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ca9 RCSB], [https://www.ebi.ac.uk/pdbsum/8ca9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ca9 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/ARY_GALME ARY_GALME] Larval storage protein (LSP) which may serve as a store of amino acids for synthesis of adult proteins (Probable). Binds the A.niger cell wall component alpha-1,3-glucan, a fungal pathogen-associated molecular pattern (PAMP) that activates the host immune response (PubMed:34443685).<ref>PMID:34443685</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Plastic waste management is a pressing ecological, social, and economic challenge. The saliva of the lepidopteran Galleria mellonella larvae is capable of oxidizing and depolymerizing polyethylene in hours at room temperature. Here, we analyze by cryo-electron microscopy (cryo-EM) G. mellonella's saliva directly from the native source. The three-dimensional reconstructions reveal that the buccal secretion is mainly composed of four hexamerins belonging to the hemocyanin/phenoloxidase family, renamed Demetra, Cibeles, Ceres, and a previously unidentified factor termed Cora. Functional assays show that this factor, as its counterparts Demetra and Ceres, is also able to oxidize and degrade polyethylene. The cryo-EM data and the x-ray analysis from purified fractions show that they self-assemble primarily into three macromolecular complexes with striking structural differences that likely modulate their activity. Overall, these results establish the ground to further explore the hexamerins' functionalities, their role in vivo, and their eventual biotechnological application. | ||
| - | + | Plastic degradation by insect hexamerins: Near-atomic resolution structures of the polyethylene-degrading proteins from the wax worm saliva.,Spinola-Amilibia M, Illanes-Vicioso R, Ruiz-L Formula See Text Pez E, Colomer-Vidal P, Rodriguez FV, Peces Perez R, Arias CF, Torroba T, Sol Formula See Text M, Arias-Palomo E, Bertocchini F Sci Adv. 2023 Sep 22;9(38):eadi6813. doi: 10.1126/sciadv.adi6813. Epub 2023 Sep , 20. PMID:37729416<ref>PMID:37729416</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 8ca9" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Galleria mellonella]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Arias-Palomo E]] | ||
| + | [[Category: Spinola-Amilibia M]] | ||
Current revision
Cryo-EM structure of the Cibeles-Demetra 3:3 heterocomplex from Galleria mellonella saliva
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