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| | <StructureSection load='5l25' size='340' side='right'caption='[[5l25]], [[Resolution|resolution]] 4.11Å' scene=''> | | <StructureSection load='5l25' size='340' side='right'caption='[[5l25]], [[Resolution|resolution]] 4.11Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5l25]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5L25 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5L25 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5l25]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5L25 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5L25 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BOR1, At2g47160, T3D7.3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4.11Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5l25 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5l25 OCA], [http://pdbe.org/5l25 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5l25 RCSB], [http://www.ebi.ac.uk/pdbsum/5l25 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5l25 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5l25 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5l25 OCA], [https://pdbe.org/5l25 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5l25 RCSB], [https://www.ebi.ac.uk/pdbsum/5l25 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5l25 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/BOR1_ARATH BOR1_ARATH]] Efflux-type boron transporter for xylem loading, responsive of boron translocation from roots to shoots under boron limitation. Under boron excess, BOR1 is transferred from the plasma membrane via the endosomes to the vacuole for degradation. Boron is essential for maintaining the integrity of plants cell walls.<ref>PMID:16805739</ref> <ref>PMID:9390427</ref> | + | [https://www.uniprot.org/uniprot/BOR1_ARATH BOR1_ARATH] Efflux-type boron transporter for xylem loading, responsive of boron translocation from roots to shoots under boron limitation. Under boron excess, BOR1 is transferred from the plasma membrane via the endosomes to the vacuole for degradation. Boron is essential for maintaining the integrity of plants cell walls.<ref>PMID:16805739</ref> <ref>PMID:9390427</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Arath]] | + | [[Category: Arabidopsis thaliana]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Stroud, R M]] | + | [[Category: Stroud RM]] |
| - | [[Category: Thurtle-Schmidt, B H]] | + | [[Category: Thurtle-Schmidt BH]] |
| - | [[Category: Anion exchanger]]
| + | |
| - | [[Category: Membrane protein]]
| + | |
| - | [[Category: Slc4]]
| + | |
| - | [[Category: Transport protein]]
| + | |
| Structural highlights
Function
BOR1_ARATH Efflux-type boron transporter for xylem loading, responsive of boron translocation from roots to shoots under boron limitation. Under boron excess, BOR1 is transferred from the plasma membrane via the endosomes to the vacuole for degradation. Boron is essential for maintaining the integrity of plants cell walls.[1] [2]
Publication Abstract from PubMed
Boron is essential for plant growth because of its incorporation into plant cell walls; however, in excess it is toxic to plants. Boron transport and homeostasis in plants is regulated in part by the borate efflux transporter Bor1, a member of the solute carrier (SLC) 4 transporter family with homology to the human bicarbonate transporter Band 3. Here, we present the 4.1-A resolution crystal structure of Arabidopsis thaliana Bor1. The structure displays a dimeric architecture in which dimerization is mediated by centralized Gate domains. Comparisons with a structure of Band 3 in an outward-open state reveal that the Core domains of Bor1 have rotated inwards to achieve an occluded state. Further structural comparisons with UapA, a xanthine transporter from the nucleobase-ascorbate transporter family, show that the downward pivoting of the Core domains relative to the Gate domains may access an inward-open state. These results suggest that the SLC4, SLC26, and nucleobase-ascorbate transporter families all share an elevator transport mechanism in which alternating access is provided by Core domains that carry substrates across a membrane.
Structure of Bor1 supports an elevator transport mechanism for SLC4 anion exchangers.,Thurtle-Schmidt BH, Stroud RM Proc Natl Acad Sci U S A. 2016 Sep 6. pii: 201612603. PMID:27601653[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Miwa K, Takano J, Fujiwara T. Improvement of seed yields under boron-limiting conditions through overexpression of BOR1, a boron transporter for xylem loading, in Arabidopsis thaliana. Plant J. 2006 Jun;46(6):1084-91. PMID:16805739 doi:http://dx.doi.org/10.1111/j.1365-313X.2006.02763.x
- ↑ Noguchi K, Yasumori M, Imai T, Naito S, Matsunaga T, Oda H, Hayashi H, Chino M, Fujiwara T. bor1-1, an Arabidopsis thaliana mutant that requires a high level of boron. Plant Physiol. 1997 Nov;115(3):901-6. PMID:9390427
- ↑ Thurtle-Schmidt BH, Stroud RM. Structure of Bor1 supports an elevator transport mechanism for SLC4 anion exchangers. Proc Natl Acad Sci U S A. 2016 Sep 6. pii: 201612603. PMID:27601653 doi:http://dx.doi.org/10.1073/pnas.1612603113
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