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| | <StructureSection load='5t3t' size='340' side='right'caption='[[5t3t]], [[Resolution|resolution]] 2.20Å' scene=''> | | <StructureSection load='5t3t' size='340' side='right'caption='[[5t3t]], [[Resolution|resolution]] 2.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5t3t]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Ebov-may Ebov-may]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T3T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5T3T FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5t3t]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Ebola_virus_-_Mayinga,_Zaire,_1976 Ebola virus - Mayinga, Zaire, 1976]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T3T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5T3T FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5t3w|5t3w]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">VP30 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=128952 EBOV-May])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5t3t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t3t OCA], [https://pdbe.org/5t3t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5t3t RCSB], [https://www.ebi.ac.uk/pdbsum/5t3t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5t3t ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5t3t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t3t OCA], [http://pdbe.org/5t3t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5t3t RCSB], [http://www.ebi.ac.uk/pdbsum/5t3t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5t3t ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/NCAP_EBOZM NCAP_EBOZM]] Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid and serves as template for transcription and replication. During replication, encapsidation by NP is coupled to RNA synthesis and all replicative products are resistant to nucleases. | + | [https://www.uniprot.org/uniprot/VP30_EBOZM VP30_EBOZM] Acts as a transcription anti-termination factor immediately after transcription initiation, but does not affect transcription elongation. This function has been found to be dependent on the formation of an RNA stem-loop at the transcription start site of the first gene. Binds to RNA.<ref>PMID:12163572</ref> [https://www.uniprot.org/uniprot/NCAP_EBOZM NCAP_EBOZM] Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid and serves as template for transcription and replication. During replication, encapsidation by NP is coupled to RNA synthesis and all replicative products are resistant to nucleases. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Ebov-may]] | + | [[Category: Ebola virus - Mayinga, Zaire, 1976]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Abelson, D M]] | + | [[Category: Abelson DM]] |
| - | [[Category: Kirchdoerfer, R N]] | + | [[Category: Kirchdoerfer RN]] |
| - | [[Category: Moyer, C L]] | + | [[Category: Moyer CL]] |
| - | [[Category: Saphire, E O]] | + | [[Category: Saphire EO]] |
| - | [[Category: Co-factor]]
| + | |
| - | [[Category: Regulator]]
| + | |
| - | [[Category: Replication]]
| + | |
| - | [[Category: Transcription]]
| + | |
| - | [[Category: Viral protein]]
| + | |
| Structural highlights
Function
VP30_EBOZM Acts as a transcription anti-termination factor immediately after transcription initiation, but does not affect transcription elongation. This function has been found to be dependent on the formation of an RNA stem-loop at the transcription start site of the first gene. Binds to RNA.[1] NCAP_EBOZM Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid and serves as template for transcription and replication. During replication, encapsidation by NP is coupled to RNA synthesis and all replicative products are resistant to nucleases.
Publication Abstract from PubMed
Filoviruses are capable of causing deadly hemorrhagic fevers. All nonsegmented negative-sense RNA-virus nucleocapsids are composed of a nucleoprotein (NP), a phosphoprotein (VP35) and a polymerase (L). However, the VP30 RNA-synthesis co-factor is unique to the filoviruses. The assembly, structure, and function of the filovirus RNA replication complex remain unclear. Here, we have characterized the interactions of Ebola, Sudan and Marburg virus VP30 with NP using in vitro biochemistry, structural biology and cell-based mini-replicon assays. We have found that the VP30 C-terminal domain interacts with a short peptide in the C-terminal region of NP. Further, we have solved crystal structures of the VP30-NP complex for both Ebola and Marburg viruses. These structures reveal that a conserved, proline-rich NP peptide binds a shallow hydrophobic cleft on the VP30 C-terminal domain. Structure-guided Ebola virus VP30 mutants have altered affinities for the NP peptide. Correlation of these VP30-NP affinities with the activity for each of these mutants in a cell-based mini-replicon assay suggests that the VP30-NP interaction plays both essential and inhibitory roles in Ebola virus RNA synthesis.
The Ebola Virus VP30-NP Interaction Is a Regulator of Viral RNA Synthesis.,Kirchdoerfer RN, Moyer CL, Abelson DM, Saphire EO PLoS Pathog. 2016 Oct 18;12(10):e1005937. doi: 10.1371/journal.ppat.1005937., eCollection 2016 Oct. PMID:27755595[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Weik M, Modrof J, Klenk HD, Becker S, Muhlberger E. Ebola virus VP30-mediated transcription is regulated by RNA secondary structure formation. J Virol. 2002 Sep;76(17):8532-9. PMID:12163572
- ↑ Kirchdoerfer RN, Moyer CL, Abelson DM, Saphire EO. The Ebola Virus VP30-NP Interaction Is a Regulator of Viral RNA Synthesis. PLoS Pathog. 2016 Oct 18;12(10):e1005937. doi: 10.1371/journal.ppat.1005937., eCollection 2016 Oct. PMID:27755595 doi:http://dx.doi.org/10.1371/journal.ppat.1005937
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