1n67
From Proteopedia
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'''Clumping Factor A from Staphylococcus aureus''' | '''Clumping Factor A from Staphylococcus aureus''' | ||
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[[Category: Rajashankar, K R.]] | [[Category: Rajashankar, K R.]] | ||
[[Category: Wann, E R.]] | [[Category: Wann, E R.]] | ||
| - | [[Category: | + | [[Category: Clumping factor]] |
| - | [[Category: | + | [[Category: Dev-igg]] |
| - | [[Category: | + | [[Category: Fibrinongen-binding]] |
| - | [[Category: | + | [[Category: Igg]] |
| - | [[Category: | + | [[Category: Igsf]] |
| - | [[Category: | + | [[Category: Immunoglobulin]] |
| - | [[Category: | + | [[Category: Staphylococcus aureus]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:08:47 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 23:08, 2 May 2008
Clumping Factor A from Staphylococcus aureus
Overview
We report here the crystal structure of the minimal ligand-binding segment of the Staphylococcus aureus MSCRAMM, clumping factor A. This fibrinogen-binding segment contains two similarly folded domains. The fold observed is a new variant of the immunoglobulin motif that we have called DE-variant or the DEv-IgG fold. This subgroup includes the ligand-binding domain of the collagen-binding S.aureus MSCRAMM CNA, and many other structures previously classified as jelly rolls. Structure predictions suggest that the four fibrinogen-binding S.aureus MSCRAMMs identified so far would also contain the same DEv-IgG fold. A systematic docking search using the C-terminal region of the fibrinogen gamma-chain as a probe suggested that a hydrophobic pocket formed between the two DEv-IgG domains of the clumping factor as the ligand-binding site. Mutagenic substitution of residues Tyr256, Pro336, Tyr338 and Lys389 in the clumping factor, which are proposed to contact the terminal residues (408)AGDV(411) of the gamma-chain, resulted in proteins with no or markedly reduced affinity for fibrinogen.
About this Structure
1N67 is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.
Reference
A novel variant of the immunoglobulin fold in surface adhesins of Staphylococcus aureus: crystal structure of the fibrinogen-binding MSCRAMM, clumping factor A., Deivanayagam CC, Wann ER, Chen W, Carson M, Rajashankar KR, Hook M, Narayana SV, EMBO J. 2002 Dec 16;21(24):6660-72. PMID:12485987 Page seeded by OCA on Sat May 3 02:08:47 2008
