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| | <StructureSection load='5u1t' size='340' side='right'caption='[[5u1t]], [[Resolution|resolution]] 2.60Å' scene=''> | | <StructureSection load='5u1t' size='340' side='right'caption='[[5u1t]], [[Resolution|resolution]] 2.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5u1t]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U1T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5U1T FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5u1t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U1T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5U1T FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5u1s|5u1s]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ESP1, YGR098C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), PDS1, YDR113C, YD9727.08C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5u1t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5u1t OCA], [https://pdbe.org/5u1t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5u1t RCSB], [https://www.ebi.ac.uk/pdbsum/5u1t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5u1t ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Separase Separase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.49 3.4.22.49] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5u1t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5u1t OCA], [http://pdbe.org/5u1t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5u1t RCSB], [http://www.ebi.ac.uk/pdbsum/5u1t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5u1t ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/ESP1_YEAST ESP1_YEAST]] Caspase-like protease, which plays a central role in the chromosome segregation by cleaving the MCD1/SCC1 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by securin/PDS1 protein. It also promotes anaphase spindle elongation. A component of the FEAR (CDC14 early anaphase release) network which promotes CDC14 release from the nucleolus during early anaphase. Cleaves SLK19.<ref>PMID:10403247</ref> <ref>PMID:11149918</ref> <ref>PMID:11832211</ref> <ref>PMID:9635435</ref> [[http://www.uniprot.org/uniprot/SECU_YEAST SECU_YEAST]] Regulatory protein, which plays a central role in chromosome stability. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESP1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESP1.<ref>PMID:9635435</ref> | + | [https://www.uniprot.org/uniprot/ESP1_YEAST ESP1_YEAST] Caspase-like protease, which plays a central role in the chromosome segregation by cleaving the MCD1/SCC1 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by securin/PDS1 protein. It also promotes anaphase spindle elongation. A component of the FEAR (CDC14 early anaphase release) network which promotes CDC14 release from the nucleolus during early anaphase. Cleaves SLK19.<ref>PMID:10403247</ref> <ref>PMID:11149918</ref> <ref>PMID:11832211</ref> <ref>PMID:9635435</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Baker's yeast]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Separase]] | + | [[Category: Saccharomyces cerevisiae S288C]] |
| - | [[Category: Luo, S]] | + | [[Category: Luo S]] |
| - | [[Category: Tong, L]] | + | [[Category: Tong L]] |
| - | [[Category: Chaperon]]
| + | |
| - | [[Category: Chromosome segregation]]
| + | |
| - | [[Category: Cohesion]]
| + | |
| - | [[Category: Esp1]]
| + | |
| - | [[Category: Helical region]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Inhibition]]
| + | |
| - | [[Category: Pds1]]
| + | |
| - | [[Category: Separase-securin complex]]
| + | |
| Structural highlights
Function
ESP1_YEAST Caspase-like protease, which plays a central role in the chromosome segregation by cleaving the MCD1/SCC1 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by securin/PDS1 protein. It also promotes anaphase spindle elongation. A component of the FEAR (CDC14 early anaphase release) network which promotes CDC14 release from the nucleolus during early anaphase. Cleaves SLK19.[1] [2] [3] [4]
Publication Abstract from PubMed
Separase is a cysteine protease with a crucial role in the dissolution of cohesion among sister chromatids during chromosome segregation. In human tumours separase is overexpressed, making it a potential target for drug discovery. The protease activity of separase is strictly regulated by the inhibitor securin, which forms a tight complex with separase and may also stabilize this enzyme. Separases are large, 140-250-kilodalton enzymes, with an amino-terminal alpha-helical region and a carboxy-terminal caspase-like catalytic domain. Although crystal structures of the C-terminal two domains of separase and low-resolution electron microscopy reconstructions of the separase-securin complex have been reported, the atomic structures of full-length separase and especially the complex with securin are unknown. Here we report crystal structures at up to 2.6 A resolution of the yeast Saccharomyces cerevisiae separase-securin complex. The alpha-helical region of separase (also known as Esp1) contains four domains (I-IV), and a substrate-binding domain immediately precedes the catalytic domain and has tight associations with it. The separase-securin complex assumes a highly elongated structure. Residues 258-373 of securin (Pds1), named the separase interaction segment, are primarily in an extended conformation and traverse the entire length of separase, interacting with all of its domains. Most importantly, residues 258-269 of securin are located in the separase active site, illuminating the mechanism of inhibition. Biochemical studies confirm the structural observations and indicate that contacts outside the separase active site are crucial for stabilizing the complex, thereby defining an important function for the helical region of separase.
Molecular mechanism for the regulation of yeast separase by securin.,Luo S, Tong L Nature. 2017 Feb 9;542(7640):255-259. doi: 10.1038/nature21061. Epub 2017 Feb 1. PMID:28146474[5]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Uhlmann F, Lottspeich F, Nasmyth K. Sister-chromatid separation at anaphase onset is promoted by cleavage of the cohesin subunit Scc1. Nature. 1999 Jul 1;400(6739):37-42. PMID:10403247 doi:http://dx.doi.org/10.1038/21831
- ↑ Jensen S, Segal M, Clarke DJ, Reed SI. A novel role of the budding yeast separin Esp1 in anaphase spindle elongation: evidence that proper spindle association of Esp1 is regulated by Pds1. J Cell Biol. 2001 Jan 8;152(1):27-40. PMID:11149918
- ↑ Stegmeier F, Visintin R, Amon A. Separase, polo kinase, the kinetochore protein Slk19, and Spo12 function in a network that controls Cdc14 localization during early anaphase. Cell. 2002 Jan 25;108(2):207-20. PMID:11832211
- ↑ Ciosk R, Zachariae W, Michaelis C, Shevchenko A, Mann M, Nasmyth K. An ESP1/PDS1 complex regulates loss of sister chromatid cohesion at the metaphase to anaphase transition in yeast. Cell. 1998 Jun 12;93(6):1067-76. PMID:9635435
- ↑ Luo S, Tong L. Molecular mechanism for the regulation of yeast separase by securin. Nature. 2017 Feb 9;542(7640):255-259. doi: 10.1038/nature21061. Epub 2017 Feb 1. PMID:28146474 doi:http://dx.doi.org/10.1038/nature21061
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