|
|
| Line 3: |
Line 3: |
| | <StructureSection load='5u92' size='340' side='right'caption='[[5u92]], [[Resolution|resolution]] 2.00Å' scene=''> | | <StructureSection load='5u92' size='340' side='right'caption='[[5u92]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5u92]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Polybetes_pythagoricus Polybetes pythagoricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U92 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5U92 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5u92]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Polybetes_pythagoricus Polybetes pythagoricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U92 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5U92 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ARG:ARGININE'>ARG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ARG:ARGININE'>ARG</scene>, <scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5u8e|5u8e]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5u92 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5u92 OCA], [https://pdbe.org/5u92 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5u92 RCSB], [https://www.ebi.ac.uk/pdbsum/5u92 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5u92 ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Arginine_kinase Arginine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.3.3 2.7.3.3] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5u92 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5u92 OCA], [http://pdbe.org/5u92 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5u92 RCSB], [http://www.ebi.ac.uk/pdbsum/5u92 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5u92 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/KARG_POLPT KARG_POLPT] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 26: |
Line 26: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Arginine kinase]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| | [[Category: Polybetes pythagoricus]] | | [[Category: Polybetes pythagoricus]] |
| - | [[Category: Garcia, C F]] | + | [[Category: Garcia CF]] |
| - | [[Category: Lopez-zavala, A A]] | + | [[Category: Lopez-zavala AA]] |
| - | [[Category: Paredes-Hernandez, J]] | + | [[Category: Paredes-Hernandez J]] |
| - | [[Category: Sotelo-Mundo, R R]] | + | [[Category: Sotelo-Mundo RR]] |
| - | [[Category: Stojanoff, V]] | + | [[Category: Stojanoff V]] |
| - | [[Category: Binary complex]]
| + | |
| - | [[Category: Phosphagen metabolism]]
| + | |
| - | [[Category: Spider]]
| + | |
| - | [[Category: Transferase]]
| + | |
| Structural highlights
Function
KARG_POLPT
Publication Abstract from PubMed
Energy buffering systems are key for homeostasis during variations in energy supply. Spiders are the most important predators for insects and therefore key in terrestrial ecosystems. From biomedical interest, spiders are important for their venoms and as a source of potent allergens, such as arginine kinase (AK, EC 2.7.3.3). AK is an enzyme crucial for energy metabolism, keeping the pool of phosphagens in invertebrates, and also an allergen for humans. In this work, we studied AK from the Argentininan spider Polybetes pythagoricus (PpAK), from its complementary DNA to the crystal structure. The PpAK cDNA from muscle was cloned, and it is comprised of 1068 nucleotides that encode a 384-amino acids protein, similar to other invertebrate AKs. The apparent Michaelis-Menten kinetic constant (Km ) was 1.7 mM with a kcat of 75 s-1. Two crystal structures are presented, the apoPvAK and PpAK bound to arginine, both in the open conformation with the active site lid (residues 310-320) completely disordered. The guanidino group binding site in the apo structure appears to be organized to accept the arginine substrate. Finally, these results contribute to knowledge of mechanistic details of the function of arginine kinase.
Biochemical and structural characterization of a novel arginine kinase from the spider Polybetes pythagoricus.,Laino A, Lopez-Zavala AA, Garcia-Orozco KD, Carrasco-Miranda JS, Santana M, Stojanoff V, Sotelo-Mundo RR, Garcia CF PeerJ. 2017 Sep 11;5:e3787. doi: 10.7717/peerj.3787. eCollection 2017. PMID:28924503[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Laino A, Lopez-Zavala AA, Garcia-Orozco KD, Carrasco-Miranda JS, Santana M, Stojanoff V, Sotelo-Mundo RR, Garcia CF. Biochemical and structural characterization of a novel arginine kinase from the spider Polybetes pythagoricus. PeerJ. 2017 Sep 11;5:e3787. doi: 10.7717/peerj.3787. eCollection 2017. PMID:28924503 doi:http://dx.doi.org/10.7717/peerj.3787
|
