1n6t
From Proteopedia
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[[Image:1n6t.jpg|left|200px]] | [[Image:1n6t.jpg|left|200px]] | ||
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| - | + | {{STRUCTURE_1n6t| PDB=1n6t | SCENE= }} | |
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'''Solution Structure of the Tachykinin Peptide Neurokinin A''' | '''Solution Structure of the Tachykinin Peptide Neurokinin A''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1N6T is a [[Single protein]] structure | + | 1N6T is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N6T OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Cowsik, S M.]] | [[Category: Cowsik, S M.]] | ||
[[Category: 3 10 helix]] | [[Category: 3 10 helix]] | ||
| - | [[Category: | + | [[Category: Dpc micelle]] |
| - | [[Category: | + | [[Category: Helix]] |
| - | [[Category: | + | [[Category: Lipid induced conformation]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:10:03 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 23:10, 2 May 2008
Solution Structure of the Tachykinin Peptide Neurokinin A
Overview
The solution structure of NKA, a decapeptide of mammalian origin, has been characterized by CD spectropolarimetry and 2D proton nuclear magnetic resonance (2D 1H-NMR) spectroscopy in both aqueous and membrane mimetic solvents. Unambiguous NMR assignments of protons have been made with the aid of correlation spectroscopy (DQF-COSY and TOCSY) experiments and nuclear Overhauser effect spectroscopy (NOESY and ROESY) experiments. The distance constraints obtained from the NMR data have been utilized to generate a family of structures, which have been refined using restrained energy minimization and dynamics. These data show that in water NKA prefers to be in an extended chain conformation whereas a helical conformation is induced in the central core and the C-terminal region (D4-M10) of the peptide in the presence of perdeuterated dodecylphosphocholine (DPC) micelles, a membrane model system. Though less defined the N-terminus also displays some degree of order and a possible turn structure. The conformation adopted by NKA in the presence of DPC micelles represents a structural motif typical of neurokinin-2 selective agonists and is similar to that reported for eledoisin in hydrophobic environment.
About this Structure
1N6T is a Single protein structure. Full crystallographic information is available from OCA.
Reference
Three-dimensional structure of the mammalian tachykinin peptide neurokinin A bound to lipid micelles., Chandrashekar IR, Cowsik SM, Biophys J. 2003 Dec;85(6):4002-11. PMID:14645089 Page seeded by OCA on Sat May 3 02:10:03 2008
