1n7f

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[[Image:1n7f.jpg|left|200px]]
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{{Structure
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|PDB= 1n7f |SIZE=350|CAPTION= <scene name='initialview01'>1n7f</scene>, resolution 1.80&Aring;
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The line below this paragraph, containing "STRUCTURE_1n7f", creates the "Structure Box" on the page.
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|GENE= grip1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus])
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{{STRUCTURE_1n7f| PDB=1n7f | SCENE= }}
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|RELATEDENTRY=[[1n7e|1N7E]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n7f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n7f OCA], [http://www.ebi.ac.uk/pdbsum/1n7f PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1n7f RCSB]</span>
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'''Crystal structure of the sixth PDZ domain of GRIP1 in complex with liprin C-terminal peptide'''
'''Crystal structure of the sixth PDZ domain of GRIP1 in complex with liprin C-terminal peptide'''
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[[Category: Rho, S H.]]
[[Category: Rho, S H.]]
[[Category: Sheng, M.]]
[[Category: Sheng, M.]]
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[[Category: grip]]
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[[Category: Grip]]
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[[Category: liprin]]
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[[Category: Liprin]]
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[[Category: pdz]]
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[[Category: Pdz]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:11:14 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:26:25 2008''
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Revision as of 23:11, 2 May 2008

Image:1n7f.jpg

Template:STRUCTURE 1n7f

Crystal structure of the sixth PDZ domain of GRIP1 in complex with liprin C-terminal peptide


Overview

PDZ domains bind to short segments within target proteins in a sequence-specific fashion. Glutamate receptor-interacting protein (GRIP)/ABP family proteins contain six to seven PDZ domains and interact via the sixth PDZ domain (class II) with the C termini of various proteins including liprin-alpha. In addition the PDZ456 domain mediates the formation of homo- and heteromultimers of GRIP proteins. To better understand the structural basis of peptide recognition by a class II PDZ domain and PDZ-mediated multimerization, we determined the crystal structures of the GRIP1 PDZ6 domain alone and in complex with a synthetic C-terminal octapeptide of human liprin-alpha at resolutions of 1.5 and 1.8 A, respectively. Remarkably, unlike other class II PDZ domains, Ile-736 at alphaB5 rather than conserved Leu-732 at alphaB1 makes a direct hydrophobic contact with the side chain of the Tyr at the -2 position of the ligand. Moreover, the peptide-bound structure of PDZ6 shows a slight reorientation of helix alphaB, indicating that the second hydrophobic pocket undergoes a conformational adaptation to accommodate the bulkiness of the Tyr side chain, and forms an antiparallel dimer through an interface located at a site distal to the peptide-binding groove. This configuration may enable formation of GRIP multimers and efficient clustering of GRIP-binding proteins.

About this Structure

1N7F is a Protein complex structure of sequences from Rattus norvegicus. Full crystallographic information is available from OCA.

Reference

Crystal structure of GRIP1 PDZ6-peptide complex reveals the structural basis for class II PDZ target recognition and PDZ domain-mediated multimerization., Im YJ, Park SH, Rho SH, Lee JH, Kang GB, Sheng M, Kim E, Eom SH, J Biol Chem. 2003 Mar 7;278(10):8501-7. Epub 2002 Dec 18. PMID:12493751 Page seeded by OCA on Sat May 3 02:11:14 2008

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