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| | ==Crystal structure of channel catfish BAX== | | ==Crystal structure of channel catfish BAX== |
| - | <StructureSection load='5w63' size='340' side='right' caption='[[5w63]], [[Resolution|resolution]] 2.44Å' scene=''> | + | <StructureSection load='5w63' size='340' side='right'caption='[[5w63]], [[Resolution|resolution]] 2.44Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5w63]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Channel_catfish Channel catfish]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W63 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W63 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5w63]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ictalurus_punctatus Ictalurus punctatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W63 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5W63 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.436Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5w5x|5w5x]], [[5w5z|5w5z]], [[5w60|5w60]], [[5w61|5w61]], [[5w62|5w62]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BAX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7998 Channel catfish])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5w63 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w63 OCA], [https://pdbe.org/5w63 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5w63 RCSB], [https://www.ebi.ac.uk/pdbsum/5w63 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5w63 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5w63 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w63 OCA], [http://pdbe.org/5w63 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5w63 RCSB], [http://www.ebi.ac.uk/pdbsum/5w63 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5w63 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/E3TFZ1_ICTPU E3TFZ1_ICTPU] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Channel catfish]] | + | [[Category: Ictalurus punctatus]] |
| - | [[Category: Colman, P M]] | + | [[Category: Large Structures]] |
| - | [[Category: Czabotar, P E]] | + | [[Category: Colman PM]] |
| - | [[Category: Luo, C S]] | + | [[Category: Czabotar PE]] |
| - | [[Category: Robin, A Y]] | + | [[Category: Luo CS]] |
| - | [[Category: Apoptosis]] | + | [[Category: Robin AY]] |
| - | [[Category: Bax]]
| + | |
| - | [[Category: Catfish]]
| + | |
| - | [[Category: Monomer]]
| + | |
| Structural highlights
Function
E3TFZ1_ICTPU
Publication Abstract from PubMed
BAX and BAK are essential mediators of intrinsic apoptosis that permeabilize the mitochondrial outer membrane. BAX activation requires its translocation from cytosol to mitochondria where conformational changes cause its oligomerization. To better understand the critical step of translocation, we examined its blockade by mutation near the C terminus (P168G) or by antibody binding near the N terminus. Similarities in the crystal structures of wild-type and BAX P168G but significant other differences suggest that cytosolic BAX exists as an ensemble of conformers, and that the distribution of conformers within the ensemble determines the different functions of wild-type and mutant proteins. We also describe the structure of BAX in complex with an antibody, 3C10, that inhibits cytosolic BAX by limiting exposure of the membrane-associating helix alpha9, as does the P168G mutation. Our data for both means of BAX inhibition argue for an allosteric model of BAX regulation that derives from properties of the ensemble of conformers.
Ensemble Properties of Bax Determine Its Function.,Robin AY, Iyer S, Birkinshaw RW, Sandow J, Wardak A, Luo CS, Shi M, Webb AI, Czabotar PE, Kluck RM, Colman PM Structure. 2018 Aug 4. pii: S0969-2126(18)30252-1. doi:, 10.1016/j.str.2018.07.006. PMID:30122452[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Robin AY, Iyer S, Birkinshaw RW, Sandow J, Wardak A, Luo CS, Shi M, Webb AI, Czabotar PE, Kluck RM, Colman PM. Ensemble Properties of Bax Determine Its Function. Structure. 2018 Aug 4. pii: S0969-2126(18)30252-1. doi:, 10.1016/j.str.2018.07.006. PMID:30122452 doi:http://dx.doi.org/10.1016/j.str.2018.07.006
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