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| | <StructureSection load='5wb5' size='340' side='right'caption='[[5wb5]], [[Resolution|resolution]] 2.70Å' scene=''> | | <StructureSection load='5wb5' size='340' side='right'caption='[[5wb5]], [[Resolution|resolution]] 2.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5wb5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Leima Leima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WB5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WB5 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5wb5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Leishmania_major Leishmania major]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WB5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WB5 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">EIF4E1, LMJF_27_1620 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5664 LEIMA]), LMJF_35_3980 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5664 LEIMA])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wb5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wb5 OCA], [http://pdbe.org/5wb5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wb5 RCSB], [http://www.ebi.ac.uk/pdbsum/5wb5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wb5 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5wb5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wb5 OCA], [https://pdbe.org/5wb5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5wb5 RCSB], [https://www.ebi.ac.uk/pdbsum/5wb5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5wb5 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/E9ADE1_LEIMA E9ADE1_LEIMA] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Leima]] | + | [[Category: Leishmania major]] |
| - | [[Category: Arthanari, H]] | + | [[Category: Arthanari H]] |
| - | [[Category: Boeszoermenyi, A]] | + | [[Category: Boeszoermenyi A]] |
| - | [[Category: Leger-Abraham, M]] | + | [[Category: Leger-Abraham M]] |
| - | [[Category: Meleppattu, S]] | + | [[Category: Meleppattu S]] |
| - | [[Category: Shapira, M]] | + | [[Category: Shapira M]] |
| - | [[Category: Wagner, G]] | + | [[Category: Wagner G]] |
| - | [[Category: Zinoviev, A]] | + | [[Category: Zinoviev A]] |
| - | [[Category: Cap]]
| + | |
| - | [[Category: Initiation factor]]
| + | |
| - | [[Category: Leishmania]]
| + | |
| - | [[Category: Parasite]]
| + | |
| - | [[Category: Translation]]
| + | |
| Structural highlights
Function
E9ADE1_LEIMA
Publication Abstract from PubMed
Leishmania parasites are unicellular pathogens that are transmitted to humans through the bite of infected sandflies. Most of the regulation of their gene expression occurs post-transcriptionally, and the different patterns of gene expression required throughout the parasites' life cycle are regulated at the level of translation. Here, we report the X-ray crystal structure of the Leishmania cap-binding isoform 1, LeishIF4E-1, bound to a protein fragment of previously unknown function, Leish4E-IP1, that binds tightly to LeishIF4E-1. The molecular structure, coupled to NMR spectroscopy experiments and in vitro cap-binding assays, reveal that Leish4E-IP1 allosterically destabilizes the binding of LeishIF4E-1 to the 5' mRNA cap. We propose mechanisms through which Leish4E-IP1-mediated LeishIF4E-1 inhibition could regulate translation initiation in the human parasite.
Structural basis for LeishIF4E-1 modulation by an interacting protein in the human parasite Leishmania major.,Meleppattu S, Arthanari H, Zinoviev A, Boeszoermenyi A, Wagner G, Shapira M, Leger-Abraham M Nucleic Acids Res. 2018 Mar 19. pii: 4942471. doi: 10.1093/nar/gky194. PMID:29562352[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Meleppattu S, Arthanari H, Zinoviev A, Boeszoermenyi A, Wagner G, Shapira M, Leger-Abraham M. Structural basis for LeishIF4E-1 modulation by an interacting protein in the human parasite Leishmania major. Nucleic Acids Res. 2018 Mar 19. pii: 4942471. doi: 10.1093/nar/gky194. PMID:29562352 doi:http://dx.doi.org/10.1093/nar/gky194
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