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| | <StructureSection load='5wjh' size='340' side='right'caption='[[5wjh]], [[Resolution|resolution]] 1.63Å' scene=''> | | <StructureSection load='5wjh' size='340' side='right'caption='[[5wjh]], [[Resolution|resolution]] 1.63Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5wjh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Parengyodontium_album Parengyodontium album]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WJH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WJH FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5wjh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Parengyodontium_album Parengyodontium album]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WJH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WJH FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MHA:(CARBAMOYLMETHYL-CARBOXYMETHYL-AMINO)-ACETIC+ACID'>MHA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.63Å</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Peptidase_K Peptidase K], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.64 3.4.21.64] </span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MHA:(CARBAMOYLMETHYL-CARBOXYMETHYL-AMINO)-ACETIC+ACID'>MHA</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wjh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wjh OCA], [http://pdbe.org/5wjh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wjh RCSB], [http://www.ebi.ac.uk/pdbsum/5wjh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wjh ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5wjh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wjh OCA], [https://pdbe.org/5wjh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5wjh RCSB], [https://www.ebi.ac.uk/pdbsum/5wjh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5wjh ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/PRTK_PARAQ PRTK_PARAQ]] Hydrolyzes keratin at aromatic and hydrophobic residues. | + | [https://www.uniprot.org/uniprot/PRTK_PARAQ PRTK_PARAQ] Hydrolyzes keratin at aromatic and hydrophobic residues. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 5wjh" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 5wjh" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Proteinase 3D structures|Proteinase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
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| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| | [[Category: Parengyodontium album]] | | [[Category: Parengyodontium album]] |
| - | [[Category: Peptidase K]]
| + | [[Category: Brennan HM]] |
| - | [[Category: Brennan, H M]] | + | [[Category: Leroy L]] |
| - | [[Category: Leroy, L]] | + | [[Category: McCarthy L]] |
| - | [[Category: McCarthy, L]] | + | [[Category: Soares AS]] |
| - | [[Category: Soares, A S]] | + | |
| - | [[Category: Acoustic droplet ejection]]
| + | |
| - | [[Category: Crystal harvesting]]
| + | |
| - | [[Category: Drug discovery]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| Structural highlights
Function
PRTK_PARAQ Hydrolyzes keratin at aromatic and hydrophobic residues.
Publication Abstract from PubMed
Crystal harvesting has proven to be difficult to automate and remains the rate-limiting step for many structure-determination and high-throughput screening projects. This has resulted in crystals being prepared more rapidly than they can be harvested for X-ray data collection. Fourth-generation synchrotrons will support extraordinarily rapid rates of data acquisition, putting further pressure on the crystal-harvesting bottleneck. Here, a simple solution is reported in which crystals can be acoustically harvested from slightly modified MiTeGen In Situ-1 crystallization plates. This technique uses an acoustic pulse to eject each crystal out of its crystallization well, through a short air column and onto a micro-mesh (improving on previous work, which required separately grown crystals to be transferred before harvesting). Crystals can be individually harvested or can be serially combined with a chemical library such as a fragment library.
Using sound pulses to solve the crystal-harvesting bottleneck.,Samara YN, Brennan HM, McCarthy L, Bollard MT, Laspina D, Wlodek JM, Campos SL, Natarajan R, Gofron K, McSweeney S, Soares AS, Leroy L Acta Crystallogr D Struct Biol. 2018 Oct 1;74(Pt 10):986-999. doi:, 10.1107/S2059798318011506. Epub 2018 Oct 2. PMID:30289409[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Samara YN, Brennan HM, McCarthy L, Bollard MT, Laspina D, Wlodek JM, Campos SL, Natarajan R, Gofron K, McSweeney S, Soares AS, Leroy L. Using sound pulses to solve the crystal-harvesting bottleneck. Acta Crystallogr D Struct Biol. 2018 Oct 1;74(Pt 10):986-999. doi:, 10.1107/S2059798318011506. Epub 2018 Oct 2. PMID:30289409 doi:http://dx.doi.org/10.1107/S2059798318011506
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