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| | <StructureSection load='5wos' size='340' side='right'caption='[[5wos]], [[Resolution|resolution]] 2.45Å' scene=''> | | <StructureSection load='5wos' size='340' side='right'caption='[[5wos]], [[Resolution|resolution]] 2.45Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5wos]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Avipoxvirus_clade_b1 Avipoxvirus clade b1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WOS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WOS FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5wos]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Canarypox_virus Canarypox virus] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WOS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WOS FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CNPV058 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=44088 Avipoxvirus clade B1])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.45Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wos FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wos OCA], [http://pdbe.org/5wos PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wos RCSB], [http://www.ebi.ac.uk/pdbsum/5wos PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wos ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5wos FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wos OCA], [https://pdbe.org/5wos PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5wos RCSB], [https://www.ebi.ac.uk/pdbsum/5wos PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5wos ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/B2L11_HUMAN B2L11_HUMAN]] Induces apoptosis and anoikis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than isoform BimEL, isoform BimL and isoform BimS. Isoform Bim-gamma induces apoptosis. Isoform Bim-alpha3 induces apoptosis possibly through a caspase-mediated pathway. Isoform BimAC and isoform BimABC lack the ability to induce apoptosis.<ref>PMID:9430630</ref> <ref>PMID:11734221</ref> <ref>PMID:12019181</ref> <ref>PMID:11997495</ref> <ref>PMID:15147734</ref> <ref>PMID:15486195</ref> | + | [https://www.uniprot.org/uniprot/ARBH_CNPV ARBH_CNPV] Plays a role in the inhibition of host apoptosis. Interacts with host proapoptotic factors BAK1 and BAX to supposedly prevent their activation.<ref>PMID:29053589</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Avipoxvirus clade b1]] | + | [[Category: Canarypox virus]] |
| | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Anasir, M I]] | + | [[Category: Anasir MI]] |
| - | [[Category: Kvansakul, M]] | + | [[Category: Kvansakul M]] |
| - | [[Category: Apoptosis]]
| + | |
| - | [[Category: Avipoxvirus]]
| + | |
| - | [[Category: Bcl-2]]
| + | |
| - | [[Category: Bim]]
| + | |
| - | [[Category: Viral protein]]
| + | |
| Structural highlights
Function
ARBH_CNPV Plays a role in the inhibition of host apoptosis. Interacts with host proapoptotic factors BAK1 and BAX to supposedly prevent their activation.[1]
Publication Abstract from PubMed
Programmed cell death or apoptosis is an important component of host defense systems against viral infection. The B-cell lymphoma 2 (Bcl-2) proteins family is the main arbiter of mitochondrially mediated apoptosis, and viruses have evolved sequence and structural mimics of Bcl-2 to subvert premature host cell apoptosis in response to viral infection. The sequencing of the canarypox virus genome identified a putative pro-survival Bcl-2 protein, CNP058. However, a role in apoptosis inhibition for CNP058 has not been identified to date. Here, we report that CNP058 is able to bind several host cell pro-death Bcl-2 proteins, including Bak and Bax, as well as several BH3 only-proteins including Bim, Bid, Bmf, Noxa, Puma, and Hrk with high to moderate affinities. We then defined the structural basis for CNP058 binding to pro-death Bcl-2 proteins by determining the crystal structure of CNP058 bound to Bim BH3. CNP058 adopts the conserved Bcl-2 like fold observed in cellular pro-survival Bcl-2 proteins, and utilizes the canonical ligand binding groove to bind Bim BH3. We then demonstrate that CNP058 is a potent inhibitor of ultraviolet (UV) induced apoptosis in a cell culture model. Our findings suggest that CNP058 is a potent inhibitor of apoptosis that is able to bind to BH3 domain peptides from a broad range of pro-death Bcl-2 proteins, and may play a key role in countering premature host apoptosis.
Structural and Functional Insight into Canarypox Virus CNP058 Mediated Regulation of Apoptosis.,Anasir MI, Baxter AA, Poon IKH, Hulett MD, Kvansakul M Viruses. 2017 Oct 20;9(10). pii: E305. doi: 10.3390/v9100305. PMID:29053589[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Anasir MI, Baxter AA, Poon IKH, Hulett MD, Kvansakul M. Structural and Functional Insight into Canarypox Virus CNP058 Mediated Regulation of Apoptosis. Viruses. 2017 Oct 20;9(10). pii: E305. doi: 10.3390/v9100305. PMID:29053589 doi:http://dx.doi.org/10.3390/v9100305
- ↑ Anasir MI, Baxter AA, Poon IKH, Hulett MD, Kvansakul M. Structural and Functional Insight into Canarypox Virus CNP058 Mediated Regulation of Apoptosis. Viruses. 2017 Oct 20;9(10). pii: E305. doi: 10.3390/v9100305. PMID:29053589 doi:http://dx.doi.org/10.3390/v9100305
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