1n8k

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[[Image:1n8k.jpg|left|200px]]
[[Image:1n8k.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1n8k |SIZE=350|CAPTION= <scene name='initialview01'>1n8k</scene>, resolution 1.13&Aring;
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The line below this paragraph, containing "STRUCTURE_1n8k", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NAJ:NICOTINAMIDE-ADENINE-DINUCLEOTIDE+(ACIDIC+FORM)'>NAJ</scene>, <scene name='pdbligand=PZO:PYRAZOLE'>PZO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Alcohol_dehydrogenase Alcohol dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.1 1.1.1.1] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= ADHE M64864 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9796 Equus caballus])
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-->
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|DOMAIN=
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{{STRUCTURE_1n8k| PDB=1n8k | SCENE= }}
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|RELATEDENTRY=[[1ju9|1JU9]], [[1hld|1HLD]], [[1htb|1HTB]], [[1deh|1DEH]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n8k OCA], [http://www.ebi.ac.uk/pdbsum/1n8k PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1n8k RCSB]</span>
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}}
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'''Horse Liver Alcohol Dehydrogenase Val292Thr Mutant Complexed to NAD+ and Pyrazole'''
'''Horse Liver Alcohol Dehydrogenase Val292Thr Mutant Complexed to NAD+ and Pyrazole'''
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[[Category: Plapp, B V.]]
[[Category: Plapp, B V.]]
[[Category: Rubach, J K.]]
[[Category: Rubach, J K.]]
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[[Category: alcohol]]
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[[Category: Alcohol]]
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[[Category: dehydrogenase]]
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[[Category: Dehydrogenase]]
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[[Category: mutant]]
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[[Category: Mutant]]
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[[Category: nicotinamide coenzyme]]
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[[Category: Nicotinamide coenzyme]]
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[[Category: pyrazole]]
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[[Category: Pyrazole]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:13:38 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:26:53 2008''
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Revision as of 23:13, 2 May 2008

Image:1n8k.jpg

Template:STRUCTURE 1n8k

Horse Liver Alcohol Dehydrogenase Val292Thr Mutant Complexed to NAD+ and Pyrazole


Overview

Amino acid residues Thr-178, Val-203, and Val-292, which interact with the nicotinamide ring of the coenzyme bound to alcohol dehydrogenase (ADH), may facilitate hydride transfer and hydrogen tunneling by orientation and dynamic effects. The T178S, T178V, V203A, V292A, V292S, and V292T substitutions significantly alter the steady state and transient kinetics of the enzyme. The V292A, V292S, and V292T enzymes have decreased affinity for coenzyme (NAD+ by 30-50-fold and NADH by 35-75-fold) as compared to the wild-type enzyme. The substitutions in the nicotinamide binding site decrease the rate constant of hydride transfer for benzyl alcohol oxidation by 3-fold (for V292T ADH) to 16-fold (for V203A ADH). The modest effects suggest that catalysis does not depend critically on individual residues and that several residues in the nicotinamide binding site contribute to catalysis. The structures of the V292T ADH-NAD+-pyrazole and wild-type ADH-NAD+-4-iodopyrazole ternary complexes are very similar. Only subtle changes in the V292T enzyme cause the large changes in coenzyme binding and the small change in hydride transfer. In these complexes, one pyrazole nitrogen binds to the catalytic zinc, and the other nitrogen forms a partial covalent bond with C4 of the nicotinamide ring, which adopts a boat conformation that is postulated to be relevant for hydride transfer. The results provide an experimental basis for evaluating the contributions of dynamics to hydride transfer.

About this Structure

1N8K is a Single protein structure of sequence from Equus caballus. Full crystallographic information is available from OCA.

Reference

Amino acid residues in the nicotinamide binding site contribute to catalysis by horse liver alcohol dehydrogenase., Rubach JK, Plapp BV, Biochemistry. 2003 Mar 18;42(10):2907-15. PMID:12627956 Page seeded by OCA on Sat May 3 02:13:38 2008

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