1n8n

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[[Image:1n8n.gif|left|200px]]
[[Image:1n8n.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1n8n |SIZE=350|CAPTION= <scene name='initialview01'>1n8n</scene>, resolution 1.69&Aring;
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The line below this paragraph, containing "STRUCTURE_1n8n", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=AU3:GOLD+3++ION'>AU3</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Acid_phosphatase Acid phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.2 3.1.3.2] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= APHA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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-->
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|DOMAIN=
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{{STRUCTURE_1n8n| PDB=1n8n | SCENE= }}
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|RELATEDENTRY=[[1n9k|1N9K]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n8n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n8n OCA], [http://www.ebi.ac.uk/pdbsum/1n8n PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1n8n RCSB]</span>
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}}
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'''Crystal structure of the Au3+ complex of AphA class B acid phosphatase/phosphotransferase from E. coli at 1.69 A resolution'''
'''Crystal structure of the Au3+ complex of AphA class B acid phosphatase/phosphotransferase from E. coli at 1.69 A resolution'''
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[[Category: Rossolini, G M.]]
[[Category: Rossolini, G M.]]
[[Category: Thaller, M C.]]
[[Category: Thaller, M C.]]
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[[Category: class b acid phosphatase]]
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[[Category: Class b acid phosphatase]]
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[[Category: dddd acid phosphatase]]
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[[Category: Dddd acid phosphatase]]
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[[Category: metallo-enzyme]]
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[[Category: Metallo-enzyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:13:42 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:26:51 2008''
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Revision as of 23:13, 2 May 2008

Image:1n8n.gif

Template:STRUCTURE 1n8n

Crystal structure of the Au3+ complex of AphA class B acid phosphatase/phosphotransferase from E. coli at 1.69 A resolution


Overview

AphA is a periplasmic acid phosphatase of Escherichia coli belonging to class B bacterial phosphatases, which is part of the DDDD superfamily of phosphohydrolases. The crystal structure of AphA has been determined at 2.2A and its resolution extended to 1.7A on an AuCl(3) derivative. This represents the first crystal structure of a class B bacterial phosphatase. Despite the lack of sequence homology, the AphA structure reveals a haloacid dehalogenase-like fold. This finding suggests that this fold could be conserved among members of the DDDD superfamily of phosphohydrolases. The active enzyme is a homotetramer built by using an extended N-terminal arm intertwining the four monomers. The active site of the native enzyme, as prepared, hosts a magnesium ion, which can be replaced by other metal ions. The structure explains the non-specific behaviour of AphA towards substrates, while a structure-based alignment with other phosphatases provides clues about the catalytic mechanism.

About this Structure

1N8N is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

The first structure of a bacterial class B Acid phosphatase reveals further structural heterogeneity among phosphatases of the haloacid dehalogenase fold., Calderone V, Forleo C, Benvenuti M, Cristina Thaller M, Maria Rossolini G, Mangani S, J Mol Biol. 2004 Jan 16;335(3):761-73. PMID:14687572 Page seeded by OCA on Sat May 3 02:13:42 2008

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