1n8w

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[[Image:1n8w.gif|left|200px]]
[[Image:1n8w.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1n8w |SIZE=350|CAPTION= <scene name='initialview01'>1n8w</scene>, resolution 2.70&Aring;
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The line below this paragraph, containing "STRUCTURE_1n8w", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=GLV:GLYOXYLIC+ACID'>GLV</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MLT:MALATE+ION'>MLT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Malate_synthase Malate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.3.9 2.3.3.9] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= GlcB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis])
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-->
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|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd00728 malate_synt_G]</span>
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{{STRUCTURE_1n8w| PDB=1n8w | SCENE= }}
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|RELATEDENTRY=[[1n8i|1N8I]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n8w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n8w OCA], [http://www.ebi.ac.uk/pdbsum/1n8w PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1n8w RCSB]</span>
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}}
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'''Biochemical and Structural Studies of Malate Synthase from Mycobacterium tuberculosis'''
'''Biochemical and Structural Studies of Malate Synthase from Mycobacterium tuberculosis'''
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[[Category: Smith, C V.]]
[[Category: Smith, C V.]]
[[Category: TBSGC, TB Structural Genomics Consortium.]]
[[Category: TBSGC, TB Structural Genomics Consortium.]]
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[[Category: coenzyme some]]
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[[Category: Coenzyme some]]
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[[Category: glcb]]
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[[Category: Glcb]]
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[[Category: glyoxylate]]
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[[Category: Glyoxylate]]
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[[Category: glyoxylate pathway]]
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[[Category: Glyoxylate pathway]]
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[[Category: malate]]
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[[Category: Malate]]
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[[Category: malate synthase]]
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[[Category: Malate synthase]]
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[[Category: mycobacterium tuberculosis]]
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[[Category: Mycobacterium tuberculosis]]
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[[Category: protein structure initiative]]
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[[Category: Protein structure initiative]]
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[[Category: psi]]
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[[Category: Psi]]
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[[Category: structural genomic]]
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[[Category: Structural genomic]]
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[[Category: tb structural genomics consortium]]
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[[Category: Tb structural genomics consortium]]
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[[Category: tbsgc]]
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[[Category: Tbsgc]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:14:24 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:26:58 2008''
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Revision as of 23:14, 2 May 2008

Image:1n8w.gif

Template:STRUCTURE 1n8w

Biochemical and Structural Studies of Malate Synthase from Mycobacterium tuberculosis


Overview

Establishment or maintenance of a persistent infection by Mycobacterium tuberculosis requires the glyoxylate pathway. This is a bypass of the tricarboxylic acid cycle in which isocitrate lyase and malate synthase (GlcB) catalyze the net incorporation of carbon during growth of microorganisms on acetate or fatty acids as the primary carbon source. The glcB gene from M. tuberculosis, which encodes malate synthase, was cloned, and GlcB was expressed in Escherichia coli. The influence of media conditions on expression in M. tuberculosis indicated that this enzyme is regulated differentially to isocitrate lyase. Purified GlcB had K(m) values of 57 and 30 microm for its substrates glyoxylate and acetyl coenzyme A, respectively, and was inhibited by bromopyruvate, oxalate, and phosphoenolpyruvate. The GlcB structure was solved to 2.1-A resolution in the presence of glyoxylate and magnesium. We also report the structure of GlcB in complex with the products of the reaction, coenzyme A and malate, solved to 2.7-A resolution. Coenzyme A binds in a bent conformation, and the details of its interactions are described, together with implications on the enzyme mechanism.

About this Structure

1N8W is a Single protein structure of sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA.

Reference

Biochemical and structural studies of malate synthase from Mycobacterium tuberculosis., Smith CV, Huang CC, Miczak A, Russell DG, Sacchettini JC, Honer zu Bentrup K, J Biol Chem. 2003 Jan 17;278(3):1735-43. Epub 2002 Oct 21. PMID:12393860 Page seeded by OCA on Sat May 3 02:14:24 2008

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