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| | <StructureSection load='6bhc' size='340' side='right'caption='[[6bhc]], [[Resolution|resolution]] 2.30Å' scene=''> | | <StructureSection load='6bhc' size='340' side='right'caption='[[6bhc]], [[Resolution|resolution]] 2.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6bhc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BHC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BHC FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6bhc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BHC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6BHC FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PEAK1, KIAA2002 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_protein-tyrosine_kinase Non-specific protein-tyrosine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.10.2 2.7.10.2] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6bhc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bhc OCA], [https://pdbe.org/6bhc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6bhc RCSB], [https://www.ebi.ac.uk/pdbsum/6bhc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6bhc ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bhc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bhc OCA], [http://pdbe.org/6bhc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bhc RCSB], [http://www.ebi.ac.uk/pdbsum/6bhc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bhc ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/PEAK1_HUMAN PEAK1_HUMAN]] Tyrosine kinase that may play a role in cell spreading and migration on fibronectin. May directly or indirectly affect phosphorylation levels of cytoskeleton-associated proteins MAPK1/ERK and PXN.<ref>PMID:20534451</ref> | + | [https://www.uniprot.org/uniprot/PEAK1_HUMAN PEAK1_HUMAN] Tyrosine kinase that may play a role in cell spreading and migration on fibronectin. May directly or indirectly affect phosphorylation levels of cytoskeleton-associated proteins MAPK1/ERK and PXN.<ref>PMID:20534451</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Non-specific protein-tyrosine kinase]]
| + | [[Category: Boggon TJ]] |
| - | [[Category: Boggon, T J]] | + | [[Category: Ha BH]] |
| - | [[Category: Ha, B H]] | + | |
| - | [[Category: Pseudokinase]]
| + | |
| - | [[Category: Transferase]]
| + | |
| Structural highlights
Function
PEAK1_HUMAN Tyrosine kinase that may play a role in cell spreading and migration on fibronectin. May directly or indirectly affect phosphorylation levels of cytoskeleton-associated proteins MAPK1/ERK and PXN.[1]
Publication Abstract from PubMed
The pseudokinase group encompasses some 10% of protein kinases, but pseudokinases diverge from canonical kinases in key motifs. The two members of the small new kinase family 3 (NKF3) group are considered pseudokinases. These proteins, pseudopodium-enriched atypical kinase 1 (PEAK1, Sugen Kinase 269, or SgK269) and pragmin (Sugen Kinase 223 or SgK223) act as scaffolds in growth factor signaling pathways, and both contain a kinase fold with degraded kinase motifs at their C termini. These kinases may harbor regions that mediate oligomerization or control other aspects of signal transduction, but a lack of structural information has precluded detailed investigations into their functional roles. In this study, we determined the X-ray crystal structure of the PEAK1 pseudokinase domain to 2.3 A resolution. The structure revealed that the PEAK1 kinase-like domain contains a closed nucleotide-binding cleft that in this conformation may deleteriously affect nucleotide binding. Moreover, we found that N- and C-terminal extensions create a highly unusual all alpha-helical split-dimerization region, termed here the split helical dimerization (SHED) region. Sequence conservation analysis suggested that this region facilitates a dimerization mode that is conserved between PEAK1 and pragmin. Finally, we observed structural similarities between the PEAK1 SHED region and the C-terminal extension (CTE) of the Parkinson's disease-associated kinase PINK1. In summary, PEAK1's kinase cleft is occluded, and its newly identified SHED region may promote an unexpected dimerization mode. Similarities of PEAK1 with the active kinase PINK1 may reclassify the latter as a member of the NKF3 group.
The crystal structure of pseudokinase PEAK1 (Sugen Kinase 269) reveals an unusual catalytic cleft and a novel mode of kinase fold dimerization.,Ha BH, Boggon TJ J Biol Chem. 2017 Dec 6. pii: RA117.000751. doi: 10.1074/jbc.RA117.000751. PMID:29212708[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Wang Y, Kelber JA, Tran Cao HS, Cantin GT, Lin R, Wang W, Kaushal S, Bristow JM, Edgington TS, Hoffman RM, Bouvet M, Yates JR 3rd, Klemke RL. Pseudopodium-enriched atypical kinase 1 regulates the cytoskeleton and cancer progression [corrected]. Proc Natl Acad Sci U S A. 2010 Jun 15;107(24):10920-5. doi:, 10.1073/pnas.0914776107. Epub 2010 Jun 1. PMID:20534451 doi:http://dx.doi.org/10.1073/pnas.0914776107
- ↑ Ha BH, Boggon TJ. The crystal structure of pseudokinase PEAK1 (Sugen Kinase 269) reveals an unusual catalytic cleft and a novel mode of kinase fold dimerization. J Biol Chem. 2017 Dec 6. pii: RA117.000751. doi: 10.1074/jbc.RA117.000751. PMID:29212708 doi:http://dx.doi.org/10.1074/jbc.RA117.000751
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