6bi4
From Proteopedia
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<StructureSection load='6bi4' size='340' side='right'caption='[[6bi4]], [[Resolution|resolution]] 2.91Å' scene=''> | <StructureSection load='6bi4' size='340' side='right'caption='[[6bi4]], [[Resolution|resolution]] 2.91Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6bi4]] is a 4 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[6bi4]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_anthracis_str._Ames Bacillus anthracis str. Ames]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4egb 4egb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BI4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6BI4 FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.91Å</td></tr> |
| - | <tr id=' | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=PRD_900003:sucrose'>PRD_900003</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | < | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6bi4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bi4 OCA], [https://pdbe.org/6bi4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6bi4 RCSB], [https://www.ebi.ac.uk/pdbsum/6bi4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6bi4 ProSAT]</span></td></tr> |
| - | < | + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | |
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A6L7HMN5_BACAN A0A6L7HMN5_BACAN] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Many bacteria require l-rhamnose as a key cell wall component. This sugar is transferred to the cell wall using an activated donor dTDP-l-rhamnose, which is produced by the dTDP-l-rhamnose biosynthetic pathway. We determined the crystal structure of the second enzyme of this pathway dTDP-alpha-d-glucose 4,6-dehydratase (RfbB) from Bacillus anthracis. Interestingly, RfbB only crystallized in the presence of the third enzyme of the pathway RfbC; however, RfbC was not present in the crystal. Our work represents the first complete structural characterization of the four proteins of this pathway in a single Gram-positive bacterium. | ||
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| + | Structure of the Bacillus anthracis dTDP-l-rhamnose biosynthetic pathway enzyme: dTDP-alpha-d-glucose 4,6-dehydratase, RfbB.,Gokey T, Halavaty AS, Minasov G, Anderson WF, Kuhn ML J Struct Biol. 2018 May;202(2):175-181. doi: 10.1016/j.jsb.2018.01.006. Epub 2018 , Jan 10. PMID:29331609<ref>PMID:29331609</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6bi4" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
*[[DTDP-glucose 4%2C6-dehydratase|DTDP-glucose 4%2C6-dehydratase]] | *[[DTDP-glucose 4%2C6-dehydratase|DTDP-glucose 4%2C6-dehydratase]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Bacillus anthracis str. Ames]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | + | [[Category: Anderson WF]] | |
| - | [[Category: Anderson | + | [[Category: Halavaty AS]] |
| - | + | [[Category: Kuhn M]] | |
| - | [[Category: Halavaty | + | [[Category: Minasov G]] |
| - | [[Category: Kuhn | + | [[Category: Shuvalova L]] |
| - | [[Category: Minasov | + | |
| - | [[Category: Shuvalova | + | |
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Current revision
2.9 Angstrom Resolution Crystal Structure of dTDP-Glucose 4,6-dehydratase (rfbB) from Bacillus anthracis str. Ames in Complex with NAD.
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