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| | ==Crystal structure of MHC-I like protein== | | ==Crystal structure of MHC-I like protein== |
| - | <StructureSection load='6bmk' size='340' side='right' caption='[[6bmk]], [[Resolution|resolution]] 2.43Å' scene=''> | + | <StructureSection load='6bmk' size='340' side='right'caption='[[6bmk]], [[Resolution|resolution]] 2.43Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6bmk]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BMK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BMK FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6bmk]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BMK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6BMK FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=F57:(2R)-1-(decanoyloxy)-3-(phosphonooxy)propan-2-yl+octadecanoate'>F57</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.43Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Cd1d2, Cd1.2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice]), B2m ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=F57:(2R)-1-(decanoyloxy)-3-(phosphonooxy)propan-2-yl+octadecanoate'>F57</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bmk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bmk OCA], [http://pdbe.org/6bmk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bmk RCSB], [http://www.ebi.ac.uk/pdbsum/6bmk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bmk ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6bmk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bmk OCA], [https://pdbe.org/6bmk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6bmk RCSB], [https://www.ebi.ac.uk/pdbsum/6bmk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6bmk ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CD1D2_MOUSE CD1D2_MOUSE]] Antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on natural killer T-cells. [[http://www.uniprot.org/uniprot/B2MG_MOUSE B2MG_MOUSE]] Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. | + | [https://www.uniprot.org/uniprot/B2MG_MOUSE B2MG_MOUSE] Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 6bmk" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 6bmk" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Beta-2 microglobulin 3D structures|Beta-2 microglobulin 3D structures]] |
| | + | *[[CD1|CD1]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Large Structures]] |
| - | [[Category: Khandokar, Y B]] | + | [[Category: Mus musculus]] |
| - | [[Category: Nours, J Le]] | + | [[Category: Khandokar YB]] |
| - | [[Category: Rossjohn, J]] | + | [[Category: Le Nours J]] |
| - | [[Category: Antigen]] | + | [[Category: Rossjohn J]] |
| - | [[Category: Cd1d]]
| + | |
| - | [[Category: Immune system]]
| + | |
| - | [[Category: Major histocompatibility complex]]
| + | |
| - | [[Category: Mhc-i]]
| + | |
| Structural highlights
Function
B2MG_MOUSE Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
Publication Abstract from PubMed
MHC class I-like CD1 molecules have evolved to present lipid-based antigens to T cells. Differences in the antigen-binding clefts of the CD1 family members determine the conformation and size of the lipids that are presented, although the factors that shape CD1 diversity remain unclear. In mice, two homologous genes, CD1D1 and CD1D2, encode the CD1d protein, which is essential to the development and function of natural killer T (NKT) cells. However, it remains unclear whether both CD1d isoforms are equivalent in their antigen presentation capacity and functions. Here, we report that CD1d2 molecules are expressed in the thymus of some mouse strains, where they select functional type I NKT cells. Intriguingly, the T cell antigen receptor repertoire and phenotype of CD1d2-selected type I NKT cells in CD1D1(-/-) mice differed from CD1d1-selected type I NKT cells. The structures of CD1d2 in complex with endogenous lipids and a truncated acyl-chain analog of alpha-galactosylceramide revealed that its A'-pocket was restricted in size compared with CD1d1. Accordingly, CD1d2 molecules could not present glycolipid antigens with long acyl chains efficiently, favoring the presentation of short acyl chain antigens. These results indicate that the two CD1d molecules present different sets of self-antigen(s) in the mouse thymus, thereby impacting the development of invariant NKT cells.
Differing roles of CD1d2 and CD1d1 proteins in type I natural killer T cell development and function.,Sundararaj S, Zhang J, Krovi SH, Bedel R, Tuttle KD, Veerapen N, Besra GS, Khandokar Y, Praveena T, Le Nours J, Matsuda JL, Rossjohn J, Gapin L Proc Natl Acad Sci U S A. 2018 Jan 19. pii: 1716669115. doi:, 10.1073/pnas.1716669115. PMID:29351991[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Sundararaj S, Zhang J, Krovi SH, Bedel R, Tuttle KD, Veerapen N, Besra GS, Khandokar Y, Praveena T, Le Nours J, Matsuda JL, Rossjohn J, Gapin L. Differing roles of CD1d2 and CD1d1 proteins in type I natural killer T cell development and function. Proc Natl Acad Sci U S A. 2018 Jan 19. pii: 1716669115. doi:, 10.1073/pnas.1716669115. PMID:29351991 doi:http://dx.doi.org/10.1073/pnas.1716669115
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