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| | <StructureSection load='6cbq' size='340' side='right'caption='[[6cbq]], [[Resolution|resolution]] 2.80Å' scene=''> | | <StructureSection load='6cbq' size='340' side='right'caption='[[6cbq]], [[Resolution|resolution]] 2.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6cbq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CBQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CBQ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6cbq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CBQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6CBQ FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EVY:(2S)-2-hexyl-N-[(3S)-2-oxooxolan-3-yl]decanamide'>EVY</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3szt|3szt]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EVY:(2S)-2-hexyl-N-[(3S)-2-oxooxolan-3-yl]decanamide'>EVY</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">phzR, qscR, sdiA_2, CAZ03_14830, CAZ10_26210, DC19_16645, HQ52_16845, PAERUG_E15_London_28_01_14_06284, PAMH19_5306 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6cbq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cbq OCA], [https://pdbe.org/6cbq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6cbq RCSB], [https://www.ebi.ac.uk/pdbsum/6cbq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6cbq ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6cbq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cbq OCA], [http://pdbe.org/6cbq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cbq RCSB], [http://www.ebi.ac.uk/pdbsum/6cbq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cbq ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q9RMS5_PSEAI Q9RMS5_PSEAI] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Churchill, M E.A]]
| + | [[Category: Pseudomonas aeruginosa]] |
| - | [[Category: Wysoczynski-Horita, C L]]
| + | [[Category: Churchill MEA]] |
| - | [[Category: Luxr-type ahl receptor]]
| + | [[Category: Wysoczynski-Horita CL]] |
| - | [[Category: Pseudomonas aeruginosa qscr]] | + | |
| - | [[Category: Transcription]] | + | |
| - | [[Category: Transcription-agonist complex]] | + | |
| Structural highlights
Function
Q9RMS5_PSEAI
Publication Abstract from PubMed
Pseudomonas aeruginosa is an opportunistic pathogen that uses the process of quorum sensing (QS) to coordinate the expression of many virulence genes. During quorum sensing, N-acyl-homoserine lactone (AHL) signaling molecules regulate the activity of three LuxR-type transcription factors, LasR, RhlR, and QscR. To better understand P. aeruginosa QS signal reception, we examined the mechanism underlying the response of QscR to synthetic agonists and antagonists using biophysical and structural approaches. The structure of QscR bound to a synthetic agonist reveals a novel mode of ligand binding supporting a general mechanism for agonist activity. In turn, antagonists of QscR with partial agonist activity were found to destabilize and greatly impair QscR dimerization and DNA binding. These results highlight the diversity of LuxR-type receptor responses to small molecule agonists and antagonists and demonstrate the potential for chemical strategies for the selective targeting of individual quorum-sensing systems. This article is protected by copyright. All rights reserved.
Mechanism of agonism and antagonism of the Pseudomonas aeruginosa quorum sensing regulator QscR with non-native ligands.,Wysoczynski-Horita CL, Boursier ME, Hill R, Hansen K, Blackwell HE, Churchill MEA Mol Microbiol. 2018 Feb 13. doi: 10.1111/mmi.13930. PMID:29437248[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Wysoczynski-Horita CL, Boursier ME, Hill R, Hansen K, Blackwell HE, Churchill MEA. Mechanism of agonism and antagonism of the Pseudomonas aeruginosa quorum sensing regulator QscR with non-native ligands. Mol Microbiol. 2018 Feb 13. doi: 10.1111/mmi.13930. PMID:29437248 doi:http://dx.doi.org/10.1111/mmi.13930
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