|
|
| Line 3: |
Line 3: |
| | <StructureSection load='6cww' size='340' side='right'caption='[[6cww]], [[Resolution|resolution]] 1.85Å' scene=''> | | <StructureSection load='6cww' size='340' side='right'caption='[[6cww]], [[Resolution|resolution]] 1.85Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6cww]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_baa-225 Atcc baa-225]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CWW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CWW FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6cww]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Caldanaerobacter_subterraneus Caldanaerobacter subterraneus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CWW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6CWW FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=211:2,2,2-NITRILOTRIETHANOL'>211</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.851Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=4CF:4-CYANO-L-PHENYLALANINE'>4CF</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=211:2,2,2-NITRILOTRIETHANOL'>211</scene>, <scene name='pdbligand=4CF:4-CYANO-L-PHENYLALANINE'>4CF</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">XD49_1849 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=911092 ATCC BAA-225])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6cww FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cww OCA], [https://pdbe.org/6cww PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6cww RCSB], [https://www.ebi.ac.uk/pdbsum/6cww PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6cww ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6cww FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cww OCA], [http://pdbe.org/6cww PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cww RCSB], [http://www.ebi.ac.uk/pdbsum/6cww PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cww ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q8RBX6_CALS4 Q8RBX6_CALS4] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 25: |
Line 26: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc baa-225]] | + | [[Category: Caldanaerobacter subterraneus]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Brewer, S H]] | + | [[Category: Brewer SH]] |
| - | [[Category: Fowler, G D]] | + | [[Category: Fowler GD]] |
| - | [[Category: Hirn, T D]] | + | [[Category: Hirn TD]] |
| - | [[Category: Kearney, C]] | + | [[Category: Kearney C]] |
| - | [[Category: Olenginski, L T]] | + | [[Category: Olenginski LT]] |
| - | [[Category: Phillips-Piro, C M]] | + | [[Category: Phillips-Piro CM]] |
| - | [[Category: Tariq, D]] | + | [[Category: Tariq D]] |
| - | [[Category: Gas binding]]
| + | |
| - | [[Category: Hnox]]
| + | |
| - | [[Category: Nonnatural amino acid]]
| + | |
| - | [[Category: Oxygen binding]]
| + | |
| - | [[Category: Unnatural amino acid]]
| + | |
| Structural highlights
Function
Q8RBX6_CALS4
Publication Abstract from PubMed
The vibrational reporter unnatural amino acid (UAA) 4-cyano-l-phenylalanine (pCNF) was genetically incorporated individually at three sites (5, 36, and 78) in the heme protein Caldanaerobacter subterraneus H-NOX to probe local hydration environments. The UAA pCNF was incorporated site-specifically using an engineered, orthogonal tRNA synthetase in E. coli. The ability of all of the pCNF-containing H-NOX proteins to form the ferrous CO, NO, or O2 ligated and unligated states was confirmed with UV-Vis spectroscopy. The solvation state at each site of the three sites of pCNF incorporation was assessed using temperature-dependent infrared spectroscopy. Specifically, the frequency-temperature line slope (FTLS) method was utilized to show that the nitrile group at site 36 was fully solvated and the nitrile group at site 78 was de-solvated (buried) in the heme pocket. The nitrile group at site 5 was found to be partially solvated suggesting that the nitrile group was involved in moderate strength hydrogen bonds. These results were confirmed by the determination of the X-ray crystal structure of the H-NOX protein construct containing pCNF at site 5.
Exploring local solvation environments of a heme protein using the spectroscopic reporter 4-cyano-l-phenylalanine.,Kearney C, Olenginski LT, Hirn TD, Fowler GD, Tariq D, Brewer SH, Phillips-Piro CM RSC Adv. 2018 Apr 9;8(24):13503-13512. doi: 10.1039/c8ra02000k. Epub 2018 Apr 10. PMID:29780583[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Kearney C, Olenginski LT, Hirn TD, Fowler GD, Tariq D, Brewer SH, Phillips-Piro CM. Exploring local solvation environments of a heme protein using the spectroscopic reporter 4-cyano-l-phenylalanine. RSC Adv. 2018 Apr 9;8(24):13503-13512. doi: 10.1039/c8ra02000k. Epub 2018 Apr 10. PMID:29780583 doi:http://dx.doi.org/10.1039/c8ra02000k
|
