This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

5kzq

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Current revision

Metabotropic Glutamate Receptor in complex with antagonist (1~{S},2~{R},3~{S},4~{S},5~{R},6~{R})-2-azanyl-3-[[3,4-bis(fluoranyl)phenyl]sulfanylmethyl]-4-oxidanyl-bicyclo[3.1.0]hexane-2,6-dicarboxylic acid

Structural highlights

5kzq is a 1 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.8Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GRM2_HUMAN G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity. May mediate suppression of neurotransmission or may be involved in synaptogenesis or synaptic stabilization.^[1] ^[2] ^[3] ^[4]

Publication Abstract from PubMed

As part of our ongoing efforts to identify novel ligands for the metabotropic glutamate 2 and 3 (mGlu2/3) receptors, we have incorporated substitution at the C3 and C4 positions of the (1S,2R,5R,6R)-2-amino-bicyclo[3.1.0]hexane-2,6-dicarboxylic acid scaffold to generate mGlu2/3 antagonists. Exploration of this structure-activity relationship (SAR) led to the identification of (1S,2R,3S,4S,5R,6R)-2-amino-3-[(3,4-difluorophenyl)sulfanylmethyl]-4-hydroxy-bicy clo[3.1.0]hexane-2,6-dicarboxylic acid hydrochloride (LY3020371.HCl, 19f), a potent, selective, and maximally efficacious mGlu2/3 antagonist. Further characterization of compound 19f binding to the human metabotropic 2 glutamate (hmGlu2) site was established by cocrystallization of this molecule with the amino terminal domain (ATD) of the hmGlu2 receptor protein. The resulting cocrystal structure revealed the specific ligand-protein interactions, which likely explain the high affinity of 19f for this site and support its functional mGlu2 antagonist pharmacology. Further characterization of 19f in vivo demonstrated an antidepressant-like signature in the mouse forced-swim test (mFST) assay when brain levels of this compound exceeded the cellular mGlu2 IC50 value.

Discovery of (1S,2R,3S,4S,5R,6R)-2-Amino-3-[(3,4-difluorophenyl)sulfanylmethyl]-4-hydroxy-bicy clo[3.1.0]hexane-2,6-dicarboxylic Acid Hydrochloride (LY3020371.HCl): A Potent, Metabotropic Glutamate 2/3 Receptor Antagonist with Antidepressant-Like Activity.,Chappell MD, Li R, Smith SC, Dressman BA, Tromiczak EG, Tripp AE, Blanco MJ, Vetman T, Quimby SJ, Matt J, Britton TC, Fivush AM, Schkeryantz JM, Mayhugh D, Erickson JA, Bures MG, Jaramillo C, Carpintero M, Diego JE, Barberis M, Garcia-Cerrada S, Soriano JF, Antonysamy S, Atwell S, MacEwan I, Condon B, Sougias C, Wang J, Zhang A, Conners K, Groshong C, Wasserman SR, Koss JW, Witkin JM, Li X, Overshiner C, Wafford KA, Seidel W, Wang XS, Heinz BA, Swanson S, Catlow JT, Bedwell DW, Monn JA, Mitch CH, Ornstein PL J Med Chem. 2016 Dec 22;59(24):10974-10993. Epub 2016 Dec 6. PMID:28002967^[5]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Gonzalez-Maeso J, Ang RL, Yuen T, Chan P, Weisstaub NV, Lopez-Gimenez JF, Zhou M, Okawa Y, Callado LF, Milligan G, Gingrich JA, Filizola M, Meana JJ, Sealfon SC. Identification of a serotonin/glutamate receptor complex implicated in psychosis. Nature. 2008 Mar 6;452(7183):93-7. doi: 10.1038/nature06612. Epub 2008 Feb 24. PMID:18297054 doi:http://dx.doi.org/10.1038/nature06612
↑ Delille HK, Becker JM, Burkhardt S, Bleher B, Terstappen GC, Schmidt M, Meyer AH, Unger L, Marek GJ, Mezler M. Heterocomplex formation of 5-HT2A-mGlu2 and its relevance for cellular signaling cascades. Neuropharmacology. 2012 Jun;62(7):2184-91. doi: 10.1016/j.neuropharm.2012.01.010., Epub 2012 Jan 25. PMID:22300836 doi:http://dx.doi.org/10.1016/j.neuropharm.2012.01.010
↑ Moreno JL, Muguruza C, Umali A, Mortillo S, Holloway T, Pilar-Cuellar F, Mocci G, Seto J, Callado LF, Neve RL, Milligan G, Sealfon SC, Lopez-Gimenez JF, Meana JJ, Benson DL, Gonzalez-Maeso J. Identification of three residues essential for 5-hydroxytryptamine 2A-metabotropic glutamate 2 (5-HT2A.mGlu2) receptor heteromerization and its psychoactive behavioral function. J Biol Chem. 2012 Dec 28;287(53):44301-19. doi: 10.1074/jbc.M112.413161. Epub, 2012 Nov 5. PMID:23129762 doi:http://dx.doi.org/10.1074/jbc.M112.413161
↑ Flor PJ, Lindauer K, Puttner I, Ruegg D, Lukic S, Knopfel T, Kuhn R. Molecular cloning, functional expression and pharmacological characterization of the human metabotropic glutamate receptor type 2. Eur J Neurosci. 1995 Apr 1;7(4):622-9. PMID:7620613
↑ Chappell MD, Li R, Smith SC, Dressman BA, Tromiczak EG, Tripp AE, Blanco MJ, Vetman T, Quimby SJ, Matt J, Britton TC, Fivush AM, Schkeryantz JM, Mayhugh D, Erickson JA, Bures MG, Jaramillo C, Carpintero M, Diego JE, Barberis M, Garcia-Cerrada S, Soriano JF, Antonysamy S, Atwell S, MacEwan I, Condon B, Sougias C, Wang J, Zhang A, Conners K, Groshong C, Wasserman SR, Koss JW, Witkin JM, Li X, Overshiner C, Wafford KA, Seidel W, Wang XS, Heinz BA, Swanson S, Catlow JT, Bedwell DW, Monn JA, Mitch CH, Ornstein PL. Discovery of (1S,2R,3S,4S,5R,6R)-2-Amino-3-[(3,4-difluorophenyl)sulfanylmethyl]-4-hydroxy-bicy clo[3.1.0]hexane-2,6-dicarboxylic Acid Hydrochloride (LY3020371.HCl): A Potent, Metabotropic Glutamate 2/3 Receptor Antagonist with Antidepressant-Like Activity. J Med Chem. 2016 Dec 22;59(24):10974-10993. Epub 2016 Dec 6. PMID:28002967

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5kzq"

@@ Line 3: / Line 3: @@
 <StructureSection load='5kzq' size='340' side='right'caption='[[5kzq]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5kzq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KZQ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5KZQ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5kzq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KZQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5KZQ FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6YS:(1~{S},2~{R},3~{S},4~{S},5~{R},6~{R})-2-AZANYL-3-[[3,4-BIS(FLUORANYL)PHENYL]SULFANYLMETHYL]-4-OXIDANYL-BICYCLO[3.1.0]HEXANE-2,6-DICARBOXYLIC+ACID'>6YS</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5kzn|5kzn]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6YS:(1~{S},2~{R},3~{S},4~{S},5~{R},6~{R})-2-AZANYL-3-[[3,4-BIS(FLUORANYL)PHENYL]SULFANYLMETHYL]-4-OXIDANYL-BICYCLO[3.1.0]HEXANE-2,6-DICARBOXYLIC+ACID'>6YS</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GRM2, GPRC1B, MGLUR2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5kzq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kzq OCA], [https://pdbe.org/5kzq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5kzq RCSB], [https://www.ebi.ac.uk/pdbsum/5kzq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5kzq ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5kzq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kzq OCA], [http://pdbe.org/5kzq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kzq RCSB], [http://www.ebi.ac.uk/pdbsum/5kzq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kzq ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/GRM2_HUMAN GRM2_HUMAN]] G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity. May mediate suppression of neurotransmission or may be involved in synaptogenesis or synaptic stabilization.<ref>PMID:18297054</ref> <ref>PMID:22300836</ref> <ref>PMID:23129762</ref> <ref>PMID:7620613</ref>
+[https://www.uniprot.org/uniprot/GRM2_HUMAN GRM2_HUMAN] G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity. May mediate suppression of neurotransmission or may be involved in synaptogenesis or synaptic stabilization.<ref>PMID:18297054</ref> <ref>PMID:22300836</ref> <ref>PMID:23129762</ref> <ref>PMID:7620613</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 27: / Line 26: @@
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Homo sapiens]]
 [[Category: Large Structures]]
-[[Category: Antonysamy, S]]
+[[Category: Antonysamy S]]
-[[Category: Atwell, S]]
+[[Category: Atwell S]]
-[[Category: Barberis, M]]
+[[Category: Barberis M]]
-[[Category: Bedwell, D]]
+[[Category: Bedwell D]]
-[[Category: Blanco, M J]]
+[[Category: Blanco M-J]]
-[[Category: Bradley, C]]
+[[Category: Bradley C]]
-[[Category: Britton, T]]
+[[Category: Britton T]]
-[[Category: Bures, M]]
+[[Category: Bures M]]
-[[Category: Carpintero, M]]
+[[Category: Carpintero M]]
-[[Category: Catlow, J]]
+[[Category: Catlow J]]
-[[Category: Chappell, M D]]
+[[Category: Chappell MD]]
-[[Category: Condon, B]]
+[[Category: Condon B]]
-[[Category: Conners, K]]
+[[Category: Conners K]]
-[[Category: Diego, J E.de]]
+[[Category: Dressman BA]]
-[[Category: Dressman, B A]]
+[[Category: Erickson JA]]
-[[Category: Erickson, J A]]
+[[Category: Fivush AM]]
-[[Category: Fivush, A M]]
+[[Category: Garcia-Cerrada S]]
-[[Category: Garcia-Cerrada, S]]
+[[Category: Groshong C]]
-[[Category: Groshong, C]]
+[[Category: Heinz BA]]
-[[Category: Heinz, B A]]
+[[Category: Jaramillo C]]
-[[Category: Jaramillo, C]]
+[[Category: Koss JW]]
-[[Category: Koss, J W]]
+[[Category: Li R]]
-[[Category: Li, R]]
+[[Category: Li X]]
-[[Category: Li, X]]
+[[Category: MacEwan I]]
-[[Category: MacEwan, I]]
+[[Category: Matt J]]
-[[Category: Matt, J]]
+[[Category: Mayhugh D]]
-[[Category: Mayhugh, D]]
+[[Category: Mitch CH]]
-[[Category: Mitch, C H]]
+[[Category: Monn JA]]
-[[Category: Monn, J A]]
+[[Category: Ornstein P]]
-[[Category: Ornstein, P]]
+[[Category: Overshiner C]]
-[[Category: Overshiner, C]]
+[[Category: Quimby SJ]]
-[[Category: Quimby, S J]]
+[[Category: Schkeryantz JM]]
-[[Category: Schkeryantz, J M]]
+[[Category: Seidel W]]
-[[Category: Seidel, W]]
+[[Category: Smith SC]]
-[[Category: Smith, S C]]
+[[Category: Soriano JF]]
-[[Category: Soriano, J F]]
+[[Category: Swanson S]]
-[[Category: Swanson, S]]
+[[Category: Tripp AE]]
-[[Category: Tripp, A E]]
+[[Category: Tromiczak EG]]
-[[Category: Tromiczak, E G]]
+[[Category: Vetman T]]
-[[Category: Vetman, T]]
+[[Category: Wafford KA]]
-[[Category: Wafford, K A]]
+[[Category: Wang J]]
-[[Category: Wang, J]]
+[[Category: Wang X-S]]
-[[Category: Wang, X S]]
+[[Category: Wasserman SR]]
-[[Category: Wasserman, S R]]
+[[Category: Witkin JM]]
-[[Category: Witkin, J M]]
+[[Category: Zhang A]]
-[[Category: Zhang, A]]
+[[Category: De Diego JE]]
-[[Category: Antagonist]]
-[[Category: Antidepressent]]
-[[Category: Mglur2]]
-[[Category: Signaling protein]]

5kzq

From Proteopedia

Current revision

Metabotropic Glutamate Receptor in complex with antagonist (1~{S},2~{R},3~{S},4~{S},5~{R},6~{R})-2-azanyl-3-[[3,4-bis(fluoranyl)phenyl]sulfanylmethyl]-4-oxidanyl-bicyclo[3.1.0]hexane-2,6-dicarboxylic acid

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox