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| | <StructureSection load='5l7z' size='340' side='right'caption='[[5l7z]], [[Resolution|resolution]] 2.37Å' scene=''> | | <StructureSection load='5l7z' size='340' side='right'caption='[[5l7z]], [[Resolution|resolution]] 2.37Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5l7z]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5L7Z OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5L7Z FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5l7z]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5L7Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5L7Z FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">exu, CG8994 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.37Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5l7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5l7z OCA], [http://pdbe.org/5l7z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5l7z RCSB], [http://www.ebi.ac.uk/pdbsum/5l7z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5l7z ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5l7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5l7z OCA], [https://pdbe.org/5l7z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5l7z RCSB], [https://www.ebi.ac.uk/pdbsum/5l7z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5l7z ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/EXU_DROME EXU_DROME]] Ensures the proper localization of the mRNA of the bicoid gene to the anterior regions of the oocyte thus playing a fundamental role in the establishment of the polarity of the oocyte. May bind the bcd mRNA. | + | [https://www.uniprot.org/uniprot/EXU_DROME EXU_DROME] Ensures the proper localization of the mRNA of the bicoid gene to the anterior regions of the oocyte thus playing a fundamental role in the establishment of the polarity of the oocyte. May bind the bcd mRNA. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Drome]] | + | [[Category: Drosophila melanogaster]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Bono, F]] | + | [[Category: Bono F]] |
| - | [[Category: Lazzaretti, D]] | + | [[Category: Lazzaretti D]] |
| - | [[Category: Veith, K]] | + | [[Category: Veith K]] |
| - | [[Category: Exonuclease]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Pseudo nuclease]]
| + | |
| - | [[Category: Rna]]
| + | |
| - | [[Category: Rna binding protein]]
| + | |
| Structural highlights
Function
EXU_DROME Ensures the proper localization of the mRNA of the bicoid gene to the anterior regions of the oocyte thus playing a fundamental role in the establishment of the polarity of the oocyte. May bind the bcd mRNA.
Publication Abstract from PubMed
Anterior patterning in Drosophila is mediated by the localization of bicoid (bcd) mRNA at the anterior pole of the oocyte. Exuperantia (Exu) is a putative exonuclease (EXO) associated with bcd and required for its localization. We present the crystal structure of Exu, which reveals a dimeric assembly with each monomer consisting of a 3'-5' EXO-like domain and a sterile alpha motif (SAM)-like domain. The catalytic site is degenerate and inactive. Instead, the EXO-like domain mediates dimerization and RNA binding. We show that Exu binds RNA directly in vitro, that the SAM-like domain is required for RNA binding activity and that Exu binds a structured element present in the bcd 3' untranslated region with high affinity. Through structure-guided mutagenesis, we show that Exu dimerization is essential for bcd localization. Our data demonstrate that Exu is a noncanonical RNA-binding protein with EXO-SAM-like domain architecture that interacts with its target RNA as a homodimer.
The bicoid mRNA localization factor Exuperantia is an RNA-binding pseudonuclease.,Lazzaretti D, Veith K, Kramer K, Basquin C, Urlaub H, Irion U, Bono F Nat Struct Mol Biol. 2016 Jul 4. doi: 10.1038/nsmb.3254. PMID:27376588[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Lazzaretti D, Veith K, Kramer K, Basquin C, Urlaub H, Irion U, Bono F. The bicoid mRNA localization factor Exuperantia is an RNA-binding pseudonuclease. Nat Struct Mol Biol. 2016 Jul 4. doi: 10.1038/nsmb.3254. PMID:27376588 doi:http://dx.doi.org/10.1038/nsmb.3254
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