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| | <StructureSection load='5lak' size='340' side='right'caption='[[5lak]], [[Resolution|resolution]] 2.30Å' scene=''> | | <StructureSection load='5lak' size='340' side='right'caption='[[5lak]], [[Resolution|resolution]] 2.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5lak]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Cavv Cavv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LAK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LAK FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5lak]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Cavally_virus Cavally virus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LAK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LAK FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.299Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=BEZ:BENZOIC+ACID'>BEZ</scene>, <scene name='pdbligand=ECC:(4S)-4-AMINO-5-HYDROXYPENTANAMIDE'>ECC</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=BEZ:BENZOIC+ACID'>BEZ</scene>, <scene name='pdbligand=ECC:(4S)-4-AMINO-5-HYDROXYPENTANAMIDE'>ECC</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5lac|5lac]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5lak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lak OCA], [https://pdbe.org/5lak PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5lak RCSB], [https://www.ebi.ac.uk/pdbsum/5lak PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5lak ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pp1ab, CAVV_gp1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1041929 CavV])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lak OCA], [http://pdbe.org/5lak PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lak RCSB], [http://www.ebi.ac.uk/pdbsum/5lak PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lak ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/F8RL29_AMV79 F8RL29_AMV79] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Cavv]] | + | [[Category: Cavally virus]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Diederich, W E]] | + | [[Category: Synthetic construct]] |
| - | [[Category: Heine, A]] | + | [[Category: Diederich WE]] |
| - | [[Category: Kanitz, M]] | + | [[Category: Heine A]] |
| - | [[Category: Hydrolase]] | + | [[Category: Kanitz M]] |
| - | [[Category: Mesonivirus]]
| + | |
| - | [[Category: Peptide-bound]]
| + | |
| - | [[Category: Protease]]
| + | |
| Structural highlights
Function
F8RL29_AMV79
Publication Abstract from PubMed
Cavally virus (CavV) is a mosquito-borne plus-strand RNA virus in the family Mesoniviridae (order Nidovirales). We present X-ray structures for the CavV 3C-like protease (3CL(pro)), as a free enzyme and in complex with a peptide aldehyde inhibitor mimicking the P4-to-P1 residues of a natural substrate. The 3CL(pro) structure (refined to 1.94A) shows that the protein forms dimers. The monomers are comprised of N-terminal domains I and II, which adopt a chymotrypsin-like fold, and a C-terminal alpha-helical domain III. The catalytic Cys-His dyad is assisted by a complex network of interactions involving a water molecule that mediates polar contacts between the catalytic His and a conserved Asp located in the domain II-III junction and is suitably positioned to stabilize the developing positive charge of the catalytic His in the transition state during catalysis. The study also reveals the structural basis for the distinct P2 Asn-specific substrate-binding pocket of mesonivirus 3CL(pro)s.
Structural basis for catalysis and substrate specificity of a 3C-like cysteine protease from a mosquito mesonivirus.,Kanitz M, Blanck S, Heine A, Gulyaeva AA, Gorbalenya AE, Ziebuhr J, Diederich WE Virology. 2019 May 2;533:21-33. doi: 10.1016/j.virol.2019.05.001. PMID:31078932[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kanitz M, Blanck S, Heine A, Gulyaeva AA, Gorbalenya AE, Ziebuhr J, Diederich WE. Structural basis for catalysis and substrate specificity of a 3C-like cysteine protease from a mosquito mesonivirus. Virology. 2019 May 2;533:21-33. doi: 10.1016/j.virol.2019.05.001. PMID:31078932 doi:http://dx.doi.org/10.1016/j.virol.2019.05.001
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