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| | <StructureSection load='6dgm' size='340' side='right'caption='[[6dgm]], [[Resolution|resolution]] 1.49Å' scene=''> | | <StructureSection load='6dgm' size='340' side='right'caption='[[6dgm]], [[Resolution|resolution]] 1.49Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6dgm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Strpv Strpv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DGM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DGM FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6dgm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pyogenes_MGAS5005 Streptococcus pyogenes MGAS5005]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DGM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6DGM FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1GP:SN-GLYCEROL-1-PHOSPHATE'>1GP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.49Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5u9z|5u9z]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1GP:SN-GLYCEROL-1-PHOSPHATE'>1GP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SPy_0793 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=293653 STRPV])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6dgm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dgm OCA], [https://pdbe.org/6dgm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6dgm RCSB], [https://www.ebi.ac.uk/pdbsum/6dgm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6dgm ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6dgm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dgm OCA], [http://pdbe.org/6dgm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dgm RCSB], [http://www.ebi.ac.uk/pdbsum/6dgm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dgm ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q48UA4_STRPM Q48UA4_STRPM] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Strpv]] | + | [[Category: Streptococcus pyogenes MGAS5005]] |
| - | [[Category: Edgar, R J]] | + | [[Category: Edgar RJ]] |
| - | [[Category: Korotkov, K V]] | + | [[Category: Korotkov KV]] |
| - | [[Category: Korotkova, N]] | + | [[Category: Korotkova N]] |
| - | [[Category: D-glycerol-3-phosphate]]
| + | |
| - | [[Category: Glycero-1-phosphate]]
| + | |
| - | [[Category: L-glycerol 1-phosphate]]
| + | |
| - | [[Category: Lancefield group a carbohydrate]]
| + | |
| - | [[Category: Transferase]]
| + | |
| Structural highlights
Function
Q48UA4_STRPM
Publication Abstract from PubMed
Cell wall glycopolymers on the surface of Gram-positive bacteria are fundamental to bacterial physiology and infection biology. Here we identify gacH, a gene in the Streptococcus pyogenes group A carbohydrate (GAC) biosynthetic cluster, in two independent transposon library screens for its ability to confer resistance to zinc and susceptibility to the bactericidal enzyme human group IIA-secreted phospholipase A2. Subsequent structural and phylogenetic analysis of the GacH extracellular domain revealed that GacH represents an alternative class of glycerol phosphate transferase. We detected the presence of glycerol phosphate in the GAC, as well as the serotype c carbohydrate from Streptococcus mutans, which depended on the presence of the respective gacH homologs. Finally, nuclear magnetic resonance analysis of GAC confirmed that glycerol phosphate is attached to approximately 25% of the GAC N-acetylglucosamine side-chains at the C6 hydroxyl group. This previously unrecognized structural modification impacts host-pathogen interaction and has implications for vaccine design.
Discovery of glycerol phosphate modification on streptococcal rhamnose polysaccharides.,Edgar RJ, van Hensbergen VP, Ruda A, Turner AG, Deng P, Le Breton Y, El-Sayed NM, Belew AT, McIver KS, McEwan AG, Morris AJ, Lambeau G, Walker MJ, Rush JS, Korotkov KV, Widmalm G, van Sorge NM, Korotkova N Nat Chem Biol. 2019 Apr 1. pii: 10.1038/s41589-019-0251-4. doi:, 10.1038/s41589-019-0251-4. PMID:30936502[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Edgar RJ, van Hensbergen VP, Ruda A, Turner AG, Deng P, Le Breton Y, El-Sayed NM, Belew AT, McIver KS, McEwan AG, Morris AJ, Lambeau G, Walker MJ, Rush JS, Korotkov KV, Widmalm G, van Sorge NM, Korotkova N. Discovery of glycerol phosphate modification on streptococcal rhamnose polysaccharides. Nat Chem Biol. 2019 Apr 1. pii: 10.1038/s41589-019-0251-4. doi:, 10.1038/s41589-019-0251-4. PMID:30936502 doi:http://dx.doi.org/10.1038/s41589-019-0251-4
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