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| | <StructureSection load='6dgv' size='340' side='right'caption='[[6dgv]], [[Resolution|resolution]] 2.80Å' scene=''> | | <StructureSection load='6dgv' size='340' side='right'caption='[[6dgv]], [[Resolution|resolution]] 2.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6dgv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_fluorescens_liquefaciens"_flugge_1886 "bacillus fluorescens liquefaciens" flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DGV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DGV FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6dgv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_fluorescens Pseudomonas fluorescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DGV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6DGV FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6dgv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dgv OCA], [http://pdbe.org/6dgv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dgv RCSB], [http://www.ebi.ac.uk/pdbsum/6dgv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dgv ProSAT]</span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene></td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6dgv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dgv OCA], [https://pdbe.org/6dgv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6dgv RCSB], [https://www.ebi.ac.uk/pdbsum/6dgv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6dgv ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0A1B3D787_PSEFL A0A1B3D787_PSEFL] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacillus fluorescens liquefaciens flugge 1886]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Looger, L L]] | + | [[Category: Pseudomonas fluorescens]] |
| - | [[Category: Marvin, J S]] | + | [[Category: Looger LL]] |
| - | [[Category: Circularly permuted gfp]] | + | [[Category: Marvin JS]] |
| - | [[Category: Fluorescent protein]]
| + | |
| - | [[Category: Fluorescent sensor]]
| + | |
| - | [[Category: Gaba binding protein]]
| + | |
| - | [[Category: Periplasmic binding protein]]
| + | |
| - | [[Category: Superfolder]]
| + | |
| Structural highlights
Function
A0A1B3D787_PSEFL
Publication Abstract from PubMed
Current techniques for monitoring GABA (gamma-aminobutyric acid), the primary inhibitory neurotransmitter in vertebrates, cannot follow transients in intact neural circuits. To develop a GABA sensor, we applied the design principles used to create the fluorescent glutamate receptor iGluSnFR. We used a protein derived from a previously unsequenced Pseudomonas fluorescens strain and performed structure-guided mutagenesis and library screening to obtain intensity-based GABA sensing fluorescence reporter (iGABASnFR) variants. iGABASnFR is genetically encoded, detects GABA release evoked by electric stimulation of afferent fibers in acute brain slices and produces readily detectable fluorescence increases in vivo in mice and zebrafish. We applied iGABASnFR to track mitochondrial GABA content and its modulation by an anticonvulsant, swimming-evoked, GABA-mediated transmission in zebrafish cerebellum, GABA release events during interictal spikes and seizures in awake mice, and found that GABA-mediated tone decreases during isoflurane anesthesia.
A genetically encoded fluorescent sensor for in vivo imaging of GABA.,Marvin JS, Shimoda Y, Magloire V, Leite M, Kawashima T, Jensen TP, Kolb I, Knott EL, Novak O, Podgorski K, Leidenheimer NJ, Rusakov DA, Ahrens MB, Kullmann DM, Looger LL Nat Methods. 2019 Jul 15. pii: 10.1038/s41592-019-0471-2. doi:, 10.1038/s41592-019-0471-2. PMID:31308547[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Marvin JS, Shimoda Y, Magloire V, Leite M, Kawashima T, Jensen TP, Kolb I, Knott EL, Novak O, Podgorski K, Leidenheimer NJ, Rusakov DA, Ahrens MB, Kullmann DM, Looger LL. A genetically encoded fluorescent sensor for in vivo imaging of GABA. Nat Methods. 2019 Jul 15. pii: 10.1038/s41592-019-0471-2. doi:, 10.1038/s41592-019-0471-2. PMID:31308547 doi:http://dx.doi.org/10.1038/s41592-019-0471-2
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