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| | <StructureSection load='6dkc' size='340' side='right'caption='[[6dkc]], [[Resolution|resolution]] 2.90Å' scene=''> | | <StructureSection load='6dkc' size='340' side='right'caption='[[6dkc]], [[Resolution|resolution]] 2.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6dkc]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DKC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DKC FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6dkc]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_YJM789 Saccharomyces cerevisiae YJM789]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DKC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6DKC FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SCY_1694 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=307796 Baker's yeast])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cyanamide_hydratase Cyanamide hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.69 4.2.1.69] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6dkc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dkc OCA], [https://pdbe.org/6dkc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6dkc RCSB], [https://www.ebi.ac.uk/pdbsum/6dkc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6dkc ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6dkc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dkc OCA], [http://pdbe.org/6dkc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dkc RCSB], [http://www.ebi.ac.uk/pdbsum/6dkc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dkc ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A7A1Y4_YEAS7 A7A1Y4_YEAS7] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Baker's yeast]] | |
| - | [[Category: Cyanamide hydratase]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Li, J]] | + | [[Category: Saccharomyces cerevisiae YJM789]] |
| - | [[Category: Moore, S A]] | + | [[Category: Li J]] |
| - | [[Category: Xiao, W]] | + | [[Category: Moore SA]] |
| - | [[Category: Cyanamide]] | + | [[Category: Xiao W]] |
| - | [[Category: Hd-domain]]
| + | |
| - | [[Category: Hydratase]]
| + | |
| - | [[Category: Lyase]]
| + | |
| - | [[Category: Metal binding protein]]
| + | |
| - | [[Category: Zn-metalloprotein]]
| + | |
| Structural highlights
Function
A7A1Y4_YEAS7
Publication Abstract from PubMed
Cyanamide (H2N-CN) is used to break bud dormancy in woody plants and to deter alcohol use in humans. The biological effects of cyanamide in both these cases require the enzyme catalase. We previously demonstrated that Saccharomyces cerevisiae exposed to cyanamide resulted in strong induction of DDI2 gene expression. Ddi2 enzymatically hydrates cyanamide to urea and belongs to the family of HD-domain metalloenzymes (named after conserved active-site metal-binding His and Asp residues). Here, we report the X-ray structure of yeast Ddi2 to 2.6 A resolution, revealing that Ddi2 is a dimeric zinc metalloenzyme. We also confirm that Ddi2 shares structural similarity with other known HD-domain proteins. HD residues His-55, His-88, and Asp-89 coordinate the active-site zinc, and the fourth zinc ligand is a water/hydroxide molecule. Other HD domain enzymes have a second aspartate metal ligand, but in Ddi2 this residue (Thr-157) does not interact with the zinc ion. Several Ddi2 active-site point mutations exhibited reduced catalytic activity. We kinetically and structurally characterized H137N and T157V mutants of Ddi2. A cyanamide soak of the Ddi2-T157V enzyme revealed cyanamide bound directly to the Zn(2+) ion, having displaced the zinc-bound water molecule. The mode of cyanamide binding to Ddi2 resembles cyanamide binding to the active-site zinc of carbonic anhydrase, a known cyanamide hydratase. Finally, we observed that the sensitivity of ddi2Delta ddi3Delta to cyanamide was not rescued by plasmids harboring ddi2-H137N or ddi2-TI57V variants, demonstrating that yeast cells require a functioning cyanamide hydratase to overcome cyanamide-induced growth defects.
Structure of Ddi2, a highly inducible detoxifying metalloenzyme from Saccharomyces cerevisiae.,Li J, Jia Y, Lin A, Hanna M, Chelico L, Xiao W, Moore SA J Biol Chem. 2019 Jul 5;294(27):10674-10685. doi: 10.1074/jbc.RA118.006394. Epub , 2019 May 31. PMID:31152065[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Li J, Jia Y, Lin A, Hanna M, Chelico L, Xiao W, Moore SA. Structure of Ddi2, a highly inducible detoxifying metalloenzyme from Saccharomyces cerevisiae. J Biol Chem. 2019 Jul 5;294(27):10674-10685. doi: 10.1074/jbc.RA118.006394. Epub , 2019 May 31. PMID:31152065 doi:http://dx.doi.org/10.1074/jbc.RA118.006394
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