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| | <StructureSection load='6dru' size='340' side='right'caption='[[6dru]], [[Resolution|resolution]] 2.70Å' scene=''> | | <StructureSection load='6dru' size='340' side='right'caption='[[6dru]], [[Resolution|resolution]] 2.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6dru]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/A._niger A. niger]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DRU OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6DRU FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6dru]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_niger Aspergillus niger]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DRU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6DRU FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=XYS:XYLOPYRANOSE'>XYS</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ATCC64974_10330, CAN33_17365 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5061 A. niger])</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=XYS:XYLOPYRANOSE'>XYS</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6dru FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dru OCA], [http://pdbe.org/6dru PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dru RCSB], [http://www.ebi.ac.uk/pdbsum/6dru PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dru ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6dru FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dru OCA], [https://pdbe.org/6dru PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6dru RCSB], [https://www.ebi.ac.uk/pdbsum/6dru PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6dru ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/XYLA_ASPNC XYLA_ASPNC] Catalyzes the liberation of alpha-xylose from the non-reducing terminal glucose of xyloglucan oligosaccharides.<ref>PMID:22033931</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: A. niger]] | + | [[Category: Aspergillus niger]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Betancourt, M]] | + | [[Category: Betancourt M]] |
| - | [[Category: Brumm, P]] | + | [[Category: Brumm P]] |
| - | [[Category: Cao, H]] | + | [[Category: Cao H]] |
| - | [[Category: NatPro, Enzyme Discovery for Natural Product Biosynthesis]]
| + | [[Category: Phillips Jr GN]] |
| - | [[Category: Phillips, G N]] | + | [[Category: Walton JD]] |
| - | [[Category: Walton, J D]] | + | [[Category: Xu W]] |
| - | [[Category: Xu, W]] | + | |
| - | [[Category: Alpha-xylosidase]]
| + | |
| - | [[Category: Enzyme discovery for natural product biosynthesis]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Natpro]]
| + | |
| - | [[Category: PSI, Protein structure initiative]]
| + | |
| - | [[Category: Structural genomic]]
| + | |
| Structural highlights
6dru is a 2 chain structure with sequence from Aspergillus niger. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Method: | X-ray diffraction, Resolution 2.7Å |
| Ligands: | , , , , , , |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
XYLA_ASPNC Catalyzes the liberation of alpha-xylose from the non-reducing terminal glucose of xyloglucan oligosaccharides.[1]
Publication Abstract from PubMed
Glycoside hydrolase family 31 (GH31) enzymes show both highly conserved folds and catalytic residues. Yet different members of GH31 show very different substrate specificities, and it is not obvious how these specificities arise from the protein sequences. The fungal alpha-xylosidase, AxlA, was originally isolated from a commercial enzyme mixture secreted by Aspergillus niger and was reported to have potential as a catalytic component in biomass deconstruction in the biofuel industry. We report here the crystal structure of AxlA in complex with its catalytic product, a hydrolyzed xyloglucan oligosaccharide. On the basis of our new structure, we provide the structural basis for AxlA's role in xyloglucan utilization and, more importantly, a new procedure to predict and differentiate C5 vs C6 sugar specific activities based on protein sequences of the functionally diverse GH31 family enzymes.
Crystal Structure of alpha-Xylosidase from Aspergillus niger in Complex with a Hydrolyzed Xyloglucan Product and New Insights in Accurately Predicting Substrate Specificities of GH31 Family Glycosidases.,Cao H, Walton JD, Brumm P, Phillips GN Jr ACS Sustain Chem Eng. 2020 Feb 17;8(6):2540-2547. doi:, 10.1021/acssuschemeng.9b07073. Epub 2020 Jan 23. PMID:32161692[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Scott-Craig JS, Borrusch MS, Banerjee G, Harvey CM, Walton JD. Biochemical and molecular characterization of secreted α-xylosidase from Aspergillus niger. J Biol Chem. 2011 Dec 16;286(50):42848-54. PMID:22033931 doi:10.1074/jbc.M111.307397
- ↑ Cao H, Walton JD, Brumm P, Phillips GN Jr. Crystal Structure of alpha-Xylosidase from Aspergillus niger in Complex with a Hydrolyzed Xyloglucan Product and New Insights in Accurately Predicting Substrate Specificities of GH31 Family Glycosidases. ACS Sustain Chem Eng. 2020 Feb 17;8(6):2540-2547. doi:, 10.1021/acssuschemeng.9b07073. Epub 2020 Jan 23. PMID:32161692 doi:http://dx.doi.org/10.1021/acssuschemeng.9b07073
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