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| | <StructureSection load='6dsp' size='340' side='right'caption='[[6dsp]], [[Resolution|resolution]] 1.37Å' scene=''> | | <StructureSection load='6dsp' size='340' side='right'caption='[[6dsp]], [[Resolution|resolution]] 1.37Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6dsp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_saccharobutylicum_dsm_13864 Clostridium saccharobutylicum dsm 13864]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DSP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6DSP FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6dsp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_saccharobutylicum_DSM_13864 Clostridium saccharobutylicum DSM 13864]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DSP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6DSP FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PAV:(2R,4S)-2-METHYL-2,3,3,4-TETRAHYDROXYTETRAHYDROFURAN'>PAV</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.37Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lsrB, CLSA_c21430 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1345695 Clostridium saccharobutylicum DSM 13864])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PAV:(2R,4S)-2-METHYL-2,3,3,4-TETRAHYDROXYTETRAHYDROFURAN'>PAV</scene></td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6dsp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dsp OCA], [https://pdbe.org/6dsp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6dsp RCSB], [https://www.ebi.ac.uk/pdbsum/6dsp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6dsp ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6dsp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dsp OCA], [https://pdbe.org/6dsp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6dsp RCSB], [https://www.ebi.ac.uk/pdbsum/6dsp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6dsp ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/U5MRH9_CLOSA U5MRH9_CLOSA] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Clostridium saccharobutylicum dsm 13864]] | + | [[Category: Clostridium saccharobutylicum DSM 13864]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Miller, S T]] | + | [[Category: Miller ST]] |
| - | [[Category: Torcato, I M]] | + | [[Category: Torcato IM]] |
| - | [[Category: Xavier, K B]] | + | [[Category: Xavier KB]] |
| - | [[Category: Ai-2 receptor]]
| + | |
| - | [[Category: Signaling protein]]
| + | |
| Structural highlights
Function
U5MRH9_CLOSA
Publication Abstract from PubMed
Autoinducer-2 (AI-2) is unique among quorum-sensing signaling molecules, as it is produced and recognized by a wide variety of bacteria and thus facilitates interspecies communication. To date, two classes of AI-2 receptors have been identified: the LuxP-type, present in the Vibrionales, and the LsrB-type, found in a number of phylogenetically distinct bacterial families. Recently, AI-2 was shown to affect the colonization levels of a variety of bacteria in the microbiome of the mouse gut, including members of the genus Clostridium, but no AI-2 receptor had been identified in this genus. Here, we identify a noncanonical, functional LsrB-type receptor in Clostridium saccharobutylicum. This novel LsrB-like receptor is the first one reported with variations in the binding-site amino acid residues that interact with AI-2. The crystal structure of the C. saccharobutylicum receptor determined at 1.35 A resolution revealed that it binds the same form of AI-2 as the other known LsrB-type receptors, and isothermal titration calorimetry (ITC) assays showed that binding of AI-2 occurs at a submicromolar concentration. Using phylogenetic analysis, we inferred that the newly identified noncanonical LsrB receptor shares a common ancestor with known LsrB receptors and that noncanonical receptors are present in bacteria from different phyla. This led us to identify putative AI-2 receptors in bacterial species in which no receptors were known, as in bacteria belonging to the Spirochaetes and Actinobacteria phyla. Thus, this work represents a significant step toward understanding how AI-2-mediated quorum sensing influences bacterial interactions in complex biological niches.
Identification of novel autoinducer-2 receptors in Clostridia reveals plasticity in the binding site of the LsrB receptor family.,Torcato IM, Kasal MR, Brito PH, Miller ST, Xavier KB J Biol Chem. 2019 Mar 22;294(12):4450-4463. doi: 10.1074/jbc.RA118.006938. Epub, 2019 Jan 29. PMID:30696769[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Torcato IM, Kasal MR, Brito PH, Miller ST, Xavier KB. Identification of novel autoinducer-2 receptors in Clostridia reveals plasticity in the binding site of the LsrB receptor family. J Biol Chem. 2019 Mar 22;294(12):4450-4463. doi: 10.1074/jbc.RA118.006938. Epub, 2019 Jan 29. PMID:30696769 doi:http://dx.doi.org/10.1074/jbc.RA118.006938
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