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| | <StructureSection load='6ebn' size='340' side='right'caption='[[6ebn]], [[Resolution|resolution]] 1.97Å' scene=''> | | <StructureSection load='6ebn' size='340' side='right'caption='[[6ebn]], [[Resolution|resolution]] 1.97Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6ebn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Psicu Psicu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EBN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EBN FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6ebn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Psilocybe_cubensis Psilocybe cubensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EBN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6EBN FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9663111Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=LLP:(2S)-2-AMINO-6-[[3-HYDROXY-2-METHYL-5-(PHOSPHONOOXYMETHYL)PYRIDIN-4-YL]METHYLIDENEAMINO]HEXANOIC+ACID'>LLP</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=LLP:(2S)-2-AMINO-6-[[3-HYDROXY-2-METHYL-5-(PHOSPHONOOXYMETHYL)PYRIDIN-4-YL]METHYLIDENEAMINO]HEXANOIC+ACID'>LLP</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ebn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ebn OCA], [http://pdbe.org/6ebn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ebn RCSB], [http://www.ebi.ac.uk/pdbsum/6ebn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ebn ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ebn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ebn OCA], [https://pdbe.org/6ebn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ebn RCSB], [https://www.ebi.ac.uk/pdbsum/6ebn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ebn ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0A4P1LYB6_PSICU A0A4P1LYB6_PSICU] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Psicu]] | + | [[Category: Psilocybe cubensis]] |
| - | [[Category: Chun-Ting, L]] | + | [[Category: Chun-Ting L]] |
| - | [[Category: Chung, Y K]] | + | [[Category: Chung YK]] |
| - | [[Category: Pluskal, T]] | + | [[Category: Pluskal T]] |
| - | [[Category: Torrens-Spence, M P]] | + | [[Category: Torrens-Spence MP]] |
| - | [[Category: Weng, J K]] | + | [[Category: Weng JK]] |
| - | [[Category: Aromatic amino acid decarboxylase]]
| + | |
| - | [[Category: Metal binding protein]]
| + | |
| Structural highlights
Function
A0A4P1LYB6_PSICU
Publication Abstract from PubMed
Aromatic L-amino acid decarboxylases (AAADs) are a phylogenetically diverse group of enzymes responsible for the decarboxylation of aromatic amino acid substrates into their corresponding aromatic arylalkylamines. AAADs have been extensively studied in mammals and plants as they catalyze the first step in the production of neurotransmitters and bioactive phytochemicals, respectively. Unlike mammals and plants, the hallucinogenic psilocybin mushroom Psilocybe cubensis reportedly employs an unrelated phosphatidylserine-decarboxylase-like enzyme to catalyze L-tryptophan decarboxylation, the first step in psilocybin biosynthesis. To explore the origin of this chemistry in psilocybin mushroom, we generated the first de novo transcriptomes of P. cubensis and investigated several putative L-tryptophan-decarboxylase-like enzymes. We report the biochemical characterization of a noncanonical AAAD from P. cubensis (PcncAAAD) that exhibits substrate permissiveness towards L-phenylalanine, L-tyrosine, and L-tryptophan, as well as chloro-tryptophan derivatives. The crystal structure of PcncAAAD revealed the presence of a unique C-terminal appendage domain featuring a novel double-beta-barrel fold. This domain is required for PcncAAAD activity and regulates catalytic rate and thermal stability through calcium binding. PcncAAAD likely plays a role in psilocybin production in P. cubensis and offers a new tool for metabolic engineering of aromatic-amino-acid-derived natural products.
Monoamine biosynthesis via a noncanonical calcium-activatable aromatic amino acid decarboxylase in psilocybin mushroom.,Torrens-Spence MP, Liu CT, Pluskal T, Chung YK, Weng JK ACS Chem Biol. 2018 Nov 28. doi: 10.1021/acschembio.8b00821. PMID:30484626[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Torrens-Spence MP, Liu CT, Pluskal T, Chung YK, Weng JK. Monoamine biosynthesis via a noncanonical calcium-activatable aromatic amino acid decarboxylase in psilocybin mushroom. ACS Chem Biol. 2018 Nov 28. doi: 10.1021/acschembio.8b00821. PMID:30484626 doi:http://dx.doi.org/10.1021/acschembio.8b00821
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