|
|
| Line 3: |
Line 3: |
| | <StructureSection load='6mlh' size='340' side='right'caption='[[6mlh]], [[Resolution|resolution]] 1.65Å' scene=''> | | <StructureSection load='6mlh' size='340' side='right'caption='[[6mlh]], [[Resolution|resolution]] 1.65Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6mlh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"xanthomonas_citri"_(hasse_1915)_dowson_1939 "xanthomonas citri" (hasse 1915) dowson 1939]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MLH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MLH FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6mlh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Xanthomonas_citri Xanthomonas citri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MLH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6MLH FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GPM:GLUCOPYRANOSYL-1-METHYL-PHOSPHONIC+ACID'>GPM</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GPM:GLUCOPYRANOSYL-1-METHYL-PHOSPHONIC+ACID'>GPM</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">xanA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=346 "Xanthomonas citri" (Hasse 1915) Dowson 1939])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6mlh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mlh OCA], [https://pdbe.org/6mlh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6mlh RCSB], [https://www.ebi.ac.uk/pdbsum/6mlh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6mlh ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mlh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mlh OCA], [http://pdbe.org/6mlh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mlh RCSB], [http://www.ebi.ac.uk/pdbsum/6mlh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mlh ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q8PGN7_XANAC Q8PGN7_XANAC] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 23: |
Line 24: |
| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Beamer, L]] | + | [[Category: Xanthomonas citri]] |
| - | [[Category: Stiers, K]] | + | [[Category: Beamer L]] |
| - | [[Category: Carbohydrate biosynthesis]] | + | [[Category: Stiers K]] |
| - | [[Category: Enzyme]]
| + | |
| - | [[Category: Isomerase]]
| + | |
| Structural highlights
Function
Q8PGN7_XANAC
Publication Abstract from PubMed
alpha-Phosphomannomutase/phosphoglucomutase (alphaPMM/PGM) from P. aeruginosa is involved in bacterial cell wall assembly and is implicated in P. aeruginosa virulence, yet few studies have addressed alphaPMM/PGM inhibition from this important Gram-negative bacterial human pathogen. Four structurally different alpha-d-glucopyranose 1-phosphate (alphaG1P) derivatives including 1-C-fluoromethylated analogues (1-3), 1,2-cyclic phosph(on)ate analogues (4-6), isosteric methylene phosphono analogues (7 and 8), and 6-fluoro-alphaG1P (9), were synthesized and assessed as potential time-dependent or reversible alphaPMM/PGM inhibitors. The resulting kinetic data were consistent with the crystallographic structures of the highly homologous Xanthomonas citri alphaPGM with inhibitors 3 and 7-9 binding to the enzyme active site (1.65-1.9 A). These structural and kinetic insights will enhance the design of future alphaPMM/PGM inhibitors.
Inhibitory Evaluation of alphaPMM/PGM from Pseudomonas aeruginosa: Chemical Synthesis, Enzyme Kinetics, and Protein Crystallographic Study.,Zhu JS, Stiers KM, Soleimani E, Groves BR, Beamer LJ, Jakeman DL J Org Chem. 2019 Jul 16. doi: 10.1021/acs.joc.9b01305. PMID:31264865[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Zhu JS, Stiers KM, Soleimani E, Groves BR, Beamer LJ, Jakeman DL. Inhibitory Evaluation of alphaPMM/PGM from Pseudomonas aeruginosa: Chemical Synthesis, Enzyme Kinetics, and Protein Crystallographic Study. J Org Chem. 2019 Jul 16. doi: 10.1021/acs.joc.9b01305. PMID:31264865 doi:http://dx.doi.org/10.1021/acs.joc.9b01305
|
