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| | <StructureSection load='6mvf' size='340' side='right'caption='[[6mvf]], [[Resolution|resolution]] 2.55Å' scene=''> | | <StructureSection load='6mvf' size='340' side='right'caption='[[6mvf]], [[Resolution|resolution]] 2.55Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6mvf]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Faecalibacterium_prausnitzii_l2-6 Faecalibacterium prausnitzii l2-6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MVF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MVF FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6mvf]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Faecalibacterium_prausnitzii_L2-6 Faecalibacterium prausnitzii L2-6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MVF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6MVF FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.55Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">FP2_04120 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=718252 Faecalibacterium prausnitzii L2-6])</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-galactosidase Beta-galactosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.23 3.2.1.23] </span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6mvf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mvf OCA], [https://pdbe.org/6mvf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6mvf RCSB], [https://www.ebi.ac.uk/pdbsum/6mvf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6mvf ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mvf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mvf OCA], [http://pdbe.org/6mvf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mvf RCSB], [http://www.ebi.ac.uk/pdbsum/6mvf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mvf ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/D4K3H3_9FIRM D4K3H3_9FIRM] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 6mvf" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 6mvf" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Galactosidase 3D structures|Galactosidase 3D structures]] |
| | + | *[[Glucuronisidase 3D structures|Glucuronisidase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Beta-galactosidase]]
| + | [[Category: Faecalibacterium prausnitzii L2-6]] |
| - | [[Category: Faecalibacterium prausnitzii l2-6]] | + | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Pellock, S J]] | + | [[Category: Pellock SJ]] |
| - | [[Category: Redinbo, M R]] | + | [[Category: Redinbo MR]] |
| - | [[Category: Beta-glucuronidase]]
| + | |
| - | [[Category: Fmn]]
| + | |
| - | [[Category: Glycoside hydrolase]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| Structural highlights
Function
D4K3H3_9FIRM
Publication Abstract from PubMed
The human gut microbiota encodes beta-glucuronidases (GUS) that play key roles in health and disease via the metabolism of glucuronate-containing carbohydrates and drugs. Hundreds of putative bacterial GUS enzymes have been identified by metagenomic analysis of the human gut microbiome, but less than 10% have characterized structures and functions. Here we describe a set of unique gut microbial GUS enzymes that bind flavin mononucleotide (FMN). First, we show using mass spectrometry, isothermal titration calorimetry, and x-ray crystallography that a purified GUS from the gut commensal microbe Faecalibacterium prausnitzii binds to FMN on a surface groove located 30A away from the active site. Second, utilizing structural and functional data from this FMN-binding GUS, we analyzed the 279 unique GUS sequences from the human microbiome project database and identified 14 putative FMN-binding GUSs. We characterized four of these hits and solved the structure of two, the GUSs from Ruminococcus gnavus and Roseburia hominis, which confirmed that these are FMN binders. Third, binding and kinetic analysis of the FMN-binding site mutants of these five GUSs show that they utilize a conserved site to bind FMN that is not essential for GUS activity, but can affect KM. Lastly, a comprehensive structural review of the PDB reveals that the FMN-binding site employed by these enzymes is unlike any structurally characterized FMN-binders to date. These findings reveal the first instance of an FMN-binding glycoside hydrolase and suggest a potential link between FMN and carbohydrate metabolism in the human gut microbiota.
Discovery and Characterization of FMN-Binding beta-Glucuronidases in the Human Gut Microbiome.,Pellock SJ, Walton WG, Ervin SM, Torres-Rivera D, Creekmore BC, Bergan G, Dunn ZD, Li B, Tripathy A, Redinbo MR J Mol Biol. 2019 Jan 15. pii: S0022-2836(19)30011-7. doi:, 10.1016/j.jmb.2019.01.013. PMID:30658055[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Pellock SJ, Walton WG, Ervin SM, Torres-Rivera D, Creekmore BC, Bergan G, Dunn ZD, Li B, Tripathy A, Redinbo MR. Discovery and Characterization of FMN-Binding beta-Glucuronidases in the Human Gut Microbiome. J Mol Biol. 2019 Jan 15. pii: S0022-2836(19)30011-7. doi:, 10.1016/j.jmb.2019.01.013. PMID:30658055 doi:http://dx.doi.org/10.1016/j.jmb.2019.01.013
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