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| | <StructureSection load='6mwn' size='340' side='right'caption='[[6mwn]], [[Resolution|resolution]] 2.84Å' scene=''> | | <StructureSection load='6mwn' size='340' side='right'caption='[[6mwn]], [[Resolution|resolution]] 2.84Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6mwn]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MWN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MWN FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6mwn]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Hepatovirus_A Hepatovirus A] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MWN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6MWN FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.838Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mwn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mwn OCA], [http://pdbe.org/6mwn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mwn RCSB], [http://www.ebi.ac.uk/pdbsum/6mwn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mwn ProSAT]</span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene></td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6mwn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mwn OCA], [https://pdbe.org/6mwn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6mwn RCSB], [https://www.ebi.ac.uk/pdbsum/6mwn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6mwn ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | </div> | | </div> |
| | <div class="pdbe-citations 6mwn" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 6mwn" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Antibody 3D structures|Antibody 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Hepatovirus A]] |
| | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Koirala, D]] | + | [[Category: Koirala D]] |
| - | [[Category: Piccirilli, J A]] | + | [[Category: Piccirilli JA]] |
| - | [[Category: Shao, Y]] | + | [[Category: Shao Y]] |
| - | [[Category: Chaperone assisted rna crystallography]]
| + | |
| - | [[Category: Fab antibody]]
| + | |
| - | [[Category: Hepatitis a virus]]
| + | |
| - | [[Category: Ire]]
| + | |
| - | [[Category: Phage display]]
| + | |
| - | [[Category: Rna]]
| + | |
| - | [[Category: Rna-immune system complex]]
| + | |
| Structural highlights
Publication Abstract from PubMed
Picornaviral IRES elements are essential for initiating the cap-independent viral translation. However, three-dimensional structures of these elements remain elusive. Here, we report a 2.84-A resolution crystal structure of hepatitis A virus IRES domain V (dV) in complex with a synthetic antibody fragment-a crystallization chaperone. The RNA adopts a three-way junction structure, topologically organized by an adenine-rich stem-loop motif. Despite no obvious sequence homology, the dV architecture shows a striking similarity to a circularly permuted form of encephalomyocarditis virus J-K domain, suggesting a conserved strategy for organizing the domain architecture. Recurrence of the motif led us to use homology modeling tools to compute a 3-dimensional structure of the corresponding domain of foot-and-mouth disease virus, revealing an analogous domain organizing motif. The topological conservation observed among these IRESs and other viral domains implicates a structured three-way junction as an architectural scaffold to pre-organize helical domains for recruiting the translation initiation machinery.
A conserved RNA structural motif for organizing topology within picornaviral internal ribosome entry sites.,Koirala D, Shao Y, Koldobskaya Y, Fuller JR, Watkins AM, Shelke SA, Pilipenko EV, Das R, Rice PA, Piccirilli JA Nat Commun. 2019 Aug 9;10(1):3629. doi: 10.1038/s41467-019-11585-z. PMID:31399592[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Koirala D, Shao Y, Koldobskaya Y, Fuller JR, Watkins AM, Shelke SA, Pilipenko EV, Das R, Rice PA, Piccirilli JA. A conserved RNA structural motif for organizing topology within picornaviral internal ribosome entry sites. Nat Commun. 2019 Aug 9;10(1):3629. doi: 10.1038/s41467-019-11585-z. PMID:31399592 doi:http://dx.doi.org/10.1038/s41467-019-11585-z
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