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Publication Abstract from PubMed

In the many scientific endeavors that are driven by organic chemistry, unambiguous identification of small molecules is of paramount importance. Over the past 50 years, NMR and other powerful spectroscopic techniques have been developed to address this challenge. While almost all of these techniques rely on inference of connectivity, the unambiguous determination of a small molecule's structure requires X-ray and/or neutron diffraction studies. In practice, however, X-ray crystallography is rarely applied in routine organic chemistry due to intrinsic limitations of both the analytes and the technique. Here we report the use of the electron cryo-microscopy (cryoEM) method microcrystal electron diffraction (MicroED) to provide routine and unambiguous structural determination of small organic molecules. From simple powders, with minimal sample preparation, we could collect high-quality MicroED data from nanocrystals ( approximately 100 nm, approximately 10(-15) g) resulting in atomic resolution (<1 A) crystal structures in minutes.

The CryoEM Method MicroED as a Powerful Tool for Small Molecule Structure Determination.,Jones CG, Martynowycz MW, Hattne J, Fulton TJ, Stoltz BM, Rodriguez JA, Nelson HM, Gonen T ACS Cent Sci. 2018 Nov 28;4(11):1587-1592. doi: 10.1021/acscentsci.8b00760. Epub , 2018 Nov 2. PMID:30555912^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.