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| | <StructureSection load='6n03' size='340' side='right'caption='[[6n03]], [[Resolution|resolution]] 2.10Å' scene=''> | | <StructureSection load='6n03' size='340' side='right'caption='[[6n03]], [[Resolution|resolution]] 2.10Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6n03]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Phymc Phymc]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N03 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6N03 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6n03]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Physeter_catodon Physeter catodon]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N03 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6N03 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CMO:CARBON+MONOXIDE'>CMO</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6mv0|6mv0]], [[6n02|6n02]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CMO:CARBON+MONOXIDE'>CMO</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9755 PHYMC])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6n03 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n03 OCA], [https://pdbe.org/6n03 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6n03 RCSB], [https://www.ebi.ac.uk/pdbsum/6n03 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6n03 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6n03 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n03 OCA], [http://pdbe.org/6n03 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6n03 RCSB], [http://www.ebi.ac.uk/pdbsum/6n03 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6n03 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MYG_PHYCD MYG_PHYCD]] Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles. | + | [https://www.uniprot.org/uniprot/MYG_PHYMC MYG_PHYMC] Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Phymc]] | + | [[Category: Physeter catodon]] |
| - | [[Category: Besaw, J]] | + | [[Category: Besaw J]] |
| - | [[Category: Finke, A D]] | + | [[Category: Finke AD]] |
| - | [[Category: Gruner, S M]] | + | [[Category: Gruner SM]] |
| - | [[Category: Mehrabi, P]] | + | [[Category: Mehrabi P]] |
| - | [[Category: Miller, R J.D]] | + | [[Category: Miller RJD]] |
| - | [[Category: Pare-Labrosse, O]] | + | [[Category: Pare-Labrosse O]] |
| - | [[Category: Sarrachini, A]] | + | [[Category: Sarrachini A]] |
| - | [[Category: Wierman, J L]] | + | [[Category: Wierman JL]] |
| - | [[Category: Myoglobin]]
| + | |
| - | [[Category: Oxygen transport]]
| + | |
| - | [[Category: Oxygen transporter]]
| + | |
| - | [[Category: Transport protein]]
| + | |
| Structural highlights
Function
MYG_PHYMC Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles.
Publication Abstract from PubMed
A fixed-target approach to high-throughput room-temperature serial synchrotron crystallography with oscillation is described. Patterned silicon chips with microwells provide high crystal-loading density with an extremely high hit rate. The microfocus, undulator-fed beamline at CHESS, which has compound refractive optics and a fast-framing detector, was built and optimized for this experiment. The high-throughput oscillation method described here collects 1-5 degrees of data per crystal at room temperature with fast (10 degrees s(-1)) oscillation rates and translation times, giving a crystal-data collection rate of 2.5 Hz. Partial datasets collected by the oscillation method at a storage-ring source provide more complete data per crystal than still images, dramatically lowering the total number of crystals needed for a complete dataset suitable for structure solution and refinement - up to two orders of magnitude fewer being required. Thus, this method is particularly well suited to instances where crystal quantities are low. It is demonstrated, through comparison of first and last oscillation images of two systems, that dose and the effects of radiation damage can be minimized through fast rotation and low angular sweeps for each crystal.
Fixed-target serial oscillation crystallography at room temperature.,Wierman JL, Pare-Labrosse O, Sarracini A, Besaw JE, Cook MJ, Oghbaey S, Daoud H, Mehrabi P, Kriksunov I, Kuo A, Schuller DJ, Smith S, Ernst OP, Szebenyi DME, Gruner SM, Miller RJD, Finke AD IUCrJ. 2019 Feb 23;6(Pt 2):305-316. doi: 10.1107/S2052252519001453. eCollection, 2019 Mar 1. PMID:30867928[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Wierman JL, Pare-Labrosse O, Sarracini A, Besaw JE, Cook MJ, Oghbaey S, Daoud H, Mehrabi P, Kriksunov I, Kuo A, Schuller DJ, Smith S, Ernst OP, Szebenyi DME, Gruner SM, Miller RJD, Finke AD. Fixed-target serial oscillation crystallography at room temperature. IUCrJ. 2019 Feb 23;6(Pt 2):305-316. doi: 10.1107/S2052252519001453. eCollection, 2019 Mar 1. PMID:30867928 doi:http://dx.doi.org/10.1107/S2052252519001453
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