1ndl
From Proteopedia
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'''THE AWD NUCLEOTIDE DIPHOSPHATE KINASE FROM DROSOPHILA''' | '''THE AWD NUCLEOTIDE DIPHOSPHATE KINASE FROM DROSOPHILA''' | ||
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[[Category: Morera, S.]] | [[Category: Morera, S.]] | ||
[[Category: Veron, M.]] | [[Category: Veron, M.]] | ||
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| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:24:24 2008'' | |
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Revision as of 23:24, 2 May 2008
THE AWD NUCLEOTIDE DIPHOSPHATE KINASE FROM DROSOPHILA
Overview
BACKGROUND: Nucleotide diphosphate kinase (NDP kinase) is a phosphate transfer enzyme involved in cell regulation and in animal development. Drosophila NDP kinase is the product of the abnormal wing disc (awd) developmental gene, a point mutation in which can produce the killer of prune (K-pn) conditional lethal phenotype. The highly homologous mammalian genes control metastasis and a human NDP kinase acts as a transcription factor. RESULTS: The X-ray structure of the Awd protein prepared from Drosophila was solved at 2.4 A resolution by molecular replacement from the homologous Dictyostelium protein. Both are hexamers, and both have the same fold and the same active site. Subunit contacts differ as a result of sequence changes in the carboxy-terminal segment and in the loop that is the site of the K-pn mutation. CONCLUSIONS: Regulatory properties of animal NDP kinases depend on interactions with other macromolecules, such as DNA and the product of the Drosophila prune gene. The Awd structure suggests an allosteric mechanism of action of NDP kinase where DNA is the effector and the protein undergoes a major conformational change, possibly dissociating to dimers.
About this Structure
1NDL is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.
Reference
Crystal structure of the Awd nucleotide diphosphate kinase from Drosophila., Chiadmi M, Morera S, Lascu I, Dumas C, Le Bras G, Veron M, Janin J, Structure. 1993 Dec 15;1(4):283-93. PMID:8081741 Page seeded by OCA on Sat May 3 02:24:24 2008
